基于微透镜阵列的光场显微测量方法研究

与传统成像技术相比,光场成像技术能够利用光场中光线的传播方向信息,采用计算成像的方式极大地提高成像系统的景深。而传统的光学显微镜分辨率越高,景深越小。文章结合光场成像技术和传统光学显微镜,通过在显微镜一次像面插入微透镜阵列,提高显微镜景深,实现光场的显微三维测量。该系统通过单次曝光即可获得光场的四维光场信息,通过数字重聚焦技术和清晰度评价函数完成光场显微测量。实验结果表明,基于微透镜阵列的光场显微测量方法是可行的。测量系统以牺牲16倍横向分辨率为代价,将显微镜头景深提高了近100倍。

Light Field Microscopic Measurement Based on Microlens Array

Compared with the traditional imaging technology, the light field imaging technology can utilize the propagation direction information of the light in the light field, and the computational imaging method can greatly improve the depth of field of the traditional imaging system. The higher the resolution of the traditional optical microscope, the smaller the depth of field. Therefore, in this paper, the light field imaging technology and traditional optical microscope was combined by inserting a microlens array on the primary image plane of the microscope, to improve the depth of field of the microscope, and realize the three-dimensional measurement of light field microscopy. The system can obtain the four-dimensional light field information of the light field through a single exposure, complete the light field microscopic measurement through digital refocusing technology and sharpness evaluation function. The experimental results show that the light field microscopy measurement method based on the microlens array is feasible. The measurement system increases the depth of field of the microscope head by nearly 100 times at the expense of 16 times the lateral resolution.