| 植物生长调节剂对内生真菌生物合成紫杉醇的影响 |
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| 引用本文： | 李娟花,陈文强,邓百万,刘开辉,解修超,彭浩,张晓伟,张曼. 植物生长调节剂对内生真菌生物合成紫杉醇的影响[J]. 食品与生物技术学报, 2011, 30(6): 879-883 |
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| 作者姓名： | 李娟花 陈文强 邓百万 刘开辉 解修超 彭浩 张晓伟 张曼 |
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| 作者单位： | 陕西理工学院生物科学与工程学院,陕西省食药用菌工程技术研究中心,陕西汉中723000 |
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| 基金项目： | 陕西省教育厅重点科研项目(08JZ21);陕西省教育厅项目(08JK248,08JK244) |
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| 摘 要： | 研究了植物生长调节剂对Metarhizium anisopliae Tax-27产紫杉醇的影响,采用HPLC法检测.结果表明,赤霉素(GA3)质量浓度为4 mg/L、茉莉酸甲酯(MeJA)质量浓度为60umol/L、矮壮素(CCC)质量浓度为25 mg/L、6-苄氨基嘌呤(6-BA)质量浓度为4 mg/L、萘乙酸(NAA)质量浓度为4 mg/L时该菌产紫杉醇的质量浓度分别为:670.49、838.11、942.57、455.72、450.48、953.35μg/L.但GA3、MeJA和CCC对M.anisopliae Tax-27菌丝体的生长有明显的抑制作用；6-BA和NAA对菌丝体的生长均有促进作用.
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| 关 键 词： | 植物生长调节剂 内生真菌 生物合成 紫杉醇 |
Effects of Plant Growth Regulators on Taxol Biosynthesis by an Endophytic Fungus |
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| LI Juan-hu,CHE Wen-qiang,DENG Bai-wan,LIU Kai-hui,XIE Xiu-chao,PENG Hao,ZHANG Xiao-Wei and ZHANG Man. Effects of Plant Growth Regulators on Taxol Biosynthesis by an Endophytic Fungus[J]. Journal of Food Science and Biotechnology, 2011, 30(6): 879-883 |
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| Authors: | LI Juan-hu CHE Wen-qiang DENG Bai-wan LIU Kai-hui XIE Xiu-chao PENG Hao ZHANG Xiao-Wei ZHANG Man |
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| Affiliation: | LI Juan-hua,CHE Wen-qiang,DENG Bai-wan,LIU Kai-hui,XIE Xiu-chao,PENG Hao,ZHANG Xiao-Wei,ZHANG Man(School of Biological Sciences & Engineering,Shaanxi University of Technology,Shaanxi Engineering Research Center of Edible and Medicated Fungi,Hanzhong 723000,China) |
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| Abstract: | In this study,the effect of plant growth regulators on the taxol biosynthesis by Metarhizium anisopliae Tax-27 was investigated.The results showed that the titer of taxol was 670.49 μg/L,838.11μg/L,942.57μg/L,455.72 μg/L,450.48 μg/L when 4 mg/L gibberellin(GA3),60μmol/L methyl jasmonate(MeJA),25mg/L chlormequat chloride(CCC),4 mg/L 6-benzylaminopurine(6-BA),and 4 mg/L 1-naphthlcetic acid(NAA) was feed to the fermentation broth,respectively..However,the addition of GA3,MeJA,CCC inhibited the mycelial growth of M.anisopliae Tax-27,on the other hand,the addition of 6-BA and NAA significance increased the mycelia growth. |
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| Keywords: | plant growth regulators endophyte biosynthesis taxol |
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