| 一株转化茴脑生成茴香醛的菌株筛选与鉴定 |
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| 引用本文： | 梁肖仍,宋张杨,曾华贺,申佩弘,蒋承建,张敏,武波.一株转化茴脑生成茴香醛的菌株筛选与鉴定[J].食品与生物技术学报,2011,30(6):956-961. |
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| 作者姓名： | 梁肖仍 宋张杨 曾华贺 申佩弘 蒋承建 张敏 武波 |
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| 作者单位： | 1. 广西大学生命科学与技术学院,广西南宁530005;微生物及植物遗传工程教育部重点实验室,广西南宁530005;广西亚热带生物资源保护利用重点实验室,广西南宁530005
2. 微生物及植物遗传工程教育部重点实验室,广西南宁530005;广西亚热带生物资源保护利用重点实验室,广西南宁530005;广西民族大学化学与生态工程学院,广西南宁530007 |
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| 摘 要： | 以反式茴脑为惟一碳源,通过富集培养和分离纯化,从环境中筛选到320株能以反式茴脑作为惟一碳源的菌株.经薄层层析法、紫外分光光度法和气相色谱法相结合对转化液进行分析,结果获得一株相对高产茴香醛的细菌菌株WGB30.该菌最适生长温度28℃,最适pH 6.5,在复筛培养基中,30℃摇床200 r/min培养12h可达到对数期,在培养60 h时茴香醛的摩尔转化率达5.08％.综合形态学、生理生化实验、Biolog EcoPlates鉴定和16SrDNA序列分析结果,将菌株WGB30鉴定为伯克霍尔德氏菌(Burkholderia sp.).
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| 关 键 词： | 反式茴脑 茴香醛 伯克霍尔德氏菌 筛选 鉴定 |
Isolation and Characterization a Strain for Bioconversion Anethole to Anisaldehyde |
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| LIANG Xiao-reng,SONG Zhang-yang,ZENG Hua-he,SHEN Pei-hong,JIANG Cheng-jian,ZHANG Min and WU Bo.Isolation and Characterization a Strain for Bioconversion Anethole to Anisaldehyde[J].Journal of Food Science and Biotechnology,2011,30(6):956-961. |
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| Authors: | LIANG Xiao-reng SONG Zhang-yang ZENG Hua-he SHEN Pei-hong JIANG Cheng-jian ZHANG Min and WU Bo |
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| Affiliation: | LIANG Xiao-reng1,2,3,SONG Zhang-yang1,ZENG Hua-he1,SHEN Pei-hong 1,JIANG Cheng-jian1,ZHANG Min 1,WU Bo2,4(1.College of life Science and Technology,Guangxi University,Nanning 530005,China,2.Key laboratory of Microbial and Plant Genetic Engineering of Ministry of Education,3.Key Laboratory of Subtropical Bioresource Conservation and Utilization of Guangxi,4.College of Chemistry and Ecological Engineering,Guangxi University for Nationalities,Nann... |
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| Abstract: | Using trans-anethole as the sole carbon source,a strain WGB30 was screened from 320 isolates,and 50 environmental samples by thin-layer chromatography,high efficiency liquid chromatography and spectrophotometry.It was found that the optimum temperature and pH is 28 ℃ and 6.5 for this strain,respectively.One loopful of the plate culture was inoculated into 100 mL medium containing 0.5% anethole as carbon source incubated at 30 ℃ for 60 h with reciprocal shaking(200 strocks/min),the ratio conversion of anisaldehyde is up to 5.08%.According to the morphological features,the results of physiological and biochemical experiments and 16S rDNA sequence analysis,this strain was identified as Burkholderia sp. |
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| Keywords: | trans-anethole anisaldehyde Burkholderia sp isolation identification |
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