| 反相高效液相色谱法测定黄酒中的草酸 |
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| 引用本文： | 谭新勇,孙军勇,谢广发,陆健.反相高效液相色谱法测定黄酒中的草酸[J].中国酿造,2012(11):159-162. |
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| 作者姓名： | 谭新勇 孙军勇 谢广发 陆健 |
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| 作者单位： | [1]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [3]江南大学生物工程学院,江苏无锡214122 [4]中国绍兴黄酒集团有限公司国家黄酒工程技术研究中心,浙江绍兴312000 |
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| 基金项目： | 基金项目：国家重点基础研究发展计划‘973项目’（2012CB720802） |
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| 摘 要： | 该文建立了一种利用反相高效液相色谱法测定黄酒中草酸的方法,该方法以邻苯二胺为衍生剂,在高温酸性条件下与草酸反应,生成强紫外吸收化合物——2,3-二羟基喹喔啉,对其检测的最优色谱条件是:Dionex Acclaim 120 C18柱(4.6×250 mm,5μm);以甲醇和0.1mol/L乙酸铵(15:85 v/v)为流动相;流速1.2mL/min;检测波长314nm;柱温25℃。该方法测定黄酒样品中草酸的回收率为91.40%~103.70%,相对标准偏差小于9.30%。该方法简便、准确,适合测定黄酒中草酸的含量。
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| 关 键 词： | 反相高效液相色谱 黄酒 草酸 2 3-二羟基喹喔啉 |
Determination of oxalic acid in Chinese rice wine by reverse phase high performance liquid chromatography |
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| TAN Xinyong,SkIN Junyong,XIE Guangfa,LU Jian.Determination of oxalic acid in Chinese rice wine by reverse phase high performance liquid chromatography[J].China Brewing,2012(11):159-162. |
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| Authors: | TAN Xinyong SkIN Junyong XIE Guangfa LU Jian |
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| Affiliation: | 1.National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, Jiangsu, China; 2.Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; 3.School of Biotechnology, Jiangnan University, Wuxi 214122, China; 4. National Engineering Research Center for Chinese Rice Wine, China Shaoxing Rice Wine Group Co., Ltd., Shaoxing 312000, China) |
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| Abstract: | In this paper, a method of reverse phase high performance liquid chromatography （RP-HPLC） was developed to determine the oxalic acid content in Chinese rice wine. The derivatizing agent o-phenylenediamine was reacted with oxalic acid to form 2,3-dihydroxyquinoxalene, which had a strong UV absorption peak. The samples were separated on Dionex Acclaim 120 C18 column （4.6x250 mm, 5μm）, eluted with mixture of methanol and ammonium acetate 0.1 mol/L （15：85, v/v） at 1.2mL/min, and detected at 314nm, the column temperature was 25℃. The recovery of rice wine was 91.40% -103.70%, and the relative standard deviation （RSD） was less than 9.30%. This method was simple and accurate, can be used for the determi- nation of oxalic acid content in rice wine. |
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| Keywords: | reverse phase high performance liquid chromatography （RP- HPLC） Chinese rice wine oxalic acid 2 3-dihydroxyquinoxalene |
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