| 响应面法优化米曲霉降解黄水的营养条件 |
| |
| 引用本文： | 刘丹,张志才,冯凡,李佳少,李敏,庞巧霞,陈克平. 响应面法优化米曲霉降解黄水的营养条件[J]. 中国酿造, 2012, 0(11): 117-121 |
| |
| 作者姓名： | 刘丹 张志才 冯凡 李佳少 李敏 庞巧霞 陈克平 |
| |
| 作者单位： | [1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏大学生命科学研究院,江苏镇江212013 [3]北京绿科天成生物有限公司,北京102300 |
| |
| 基金项目： | 国家自然科学基金（31101269）;江苏省自然科学基金（BK2011154）;南通社会发展基金（S2011003） |
| |
| 摘 要： | 酒厂黄水中含有大量的有机物质,如直接排放会造成资源浪费和环境污染。米曲霉CGMCC5992能有效降解黄水中的有机物质。为提高其降解效率,采用单因素试验,选择影响米曲霉降解黄水的重要营养因素。并采用3因素3水平的Box-Behnken试验设计,以COD为响应值进行响应面分析,优化米曲霉降解黄水的营养条件。结果表明,最优培养基组成为10%黄水中添加0.3g/L尿素,20.73mg/L ZnSO4和19.79mg/L VB6;此条件下,在发酵第5d黄水COD值可降至323mg/L。试验证明优化后黄水的COD显著降低。
|
| 关 键 词： | 黄水 米曲霉CGMCC5992 化学需氧量(COD) Box-Behnken设计 响应面 |
Optimization of nutrition requirement for bio-degradation of vinasse by Aspergillus oryzae using response surface methodology |
| |
| LIU Dan,ZHANG Zhicai.,FENG Fan,LI Jiasha,LI Min,PANG Qiaoxia,CHEN Keping. Optimization of nutrition requirement for bio-degradation of vinasse by Aspergillus oryzae using response surface methodology[J]. China Brewing, 2012, 0(11): 117-121 |
| |
| Authors: | LIU Dan ZHANG Zhicai. FENG Fan LI Jiasha LI Min PANG Qiaoxia CHEN Keping |
| |
| Affiliation: | 1.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; 2. Institute of Life Sciences, Jiangsu University, Zbenjiang 212013, China; 3.Beijing Green Technology and Natural B iotechnology Co., Ltd., Beijing 102300, China) |
| |
| Abstract: | Direct discharge of vinasse from distillery results in resource＇ s waste and environmental destruction due to organic components in vinasse. Aspergillus oryzae CGMCC5992 is capable of degrading the organic substances of vinasse. To improve the efficiency of degradation, single-factor design was adopted to select the most important factors that influence organic materials degradation of vinasse. Box-Behnken Design （BBD） of 3 factors with 3 levels was used to optimize the degradation conditions using COD as response values. The optimum medium composition was 0.3g/L urea, 20.73mg/L ZnSO4 and 19.79mg/L VB6 withl0-times diluted vinasse. Under the conditions, the minimum COD was 323mg/L was achieved after 5d. It was demonstrated COD in vinasse was more significantly lowered after optimization. |
| |
| Keywords: | vinasse Aspergillus oryzae CGMCC5992 chemical oxygen demand Box-Behnken design response surface methodology |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
