| 血啉甲醚对单增李斯特菌的光动力灭活作用及机理 |
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| 引用本文： | 林少玲,唐书泽,唐姝姝,吴希阳,陈振强.血啉甲醚对单增李斯特菌的光动力灭活作用及机理[J].食品与发酵工业,2011,37(3). |
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| 作者姓名： | 林少玲 唐书泽 唐姝姝 吴希阳 陈振强 |
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| 作者单位： | 1. 暨南大学食品科学与工程系,广东广州,510632
2. 暨南大学光电工程系,广东广州,510632 |
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| 基金项目： | 广东省食品安全应急技术研究课题 |
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| 摘 要： | 通过平板菌落计数法研究血啉甲醚对单增李斯特菌的光动力灭活作用,同时采用聚丙烯酰胺凝胶电泳和聚合酶链式反应分析光动力灭菌技术对单增李斯特菌蛋白降解效果和基因组DNA的损伤程度。实验发现浓度为25μg/mL的血啉甲醚在光功密度为200 mW/cm2的溴钨灯光照射30 m in杀灭了99.9999%的单增李斯特菌,并导致了其蛋白质降解和基因组DNA片段断裂。血啉甲醚对单增李斯特菌的光动力灭活作用非常显著,其灭活机理可能是通过对蛋白质降解和基因组DNA损伤实现的。
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| 关 键 词： | 血啉甲醚 光动力灭菌技术 单增李斯特菌 蛋白质降解 脱氧核糖核酸损伤 |
Mechanisms of Anti-microbial Photodynamic Technology Against Listeria monocytogenes by Hematoporyrin Monomethyl Ether |
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| Lin Shao-ling,Tang Shu-ze,Tang Shu-shu,Wu Xi-yang,Chen Zhen-qiang.Mechanisms of Anti-microbial Photodynamic Technology Against Listeria monocytogenes by Hematoporyrin Monomethyl Ether[J].Food and Fermentation Industries,2011,37(3). |
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| Authors: | Lin Shao-ling Tang Shu-ze Tang Shu-shu Wu Xi-yang Chen Zhen-qiang |
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| Affiliation: | Lin Shao-ling1,Tang Shu-ze1,Tang Shu-shu1,Wu Xi-yang1,Chen Zhen-qiang21(Department of Food Science and Engineering of Jinan University,Guangzhou 510632,China) 2(Department of Optoelectronic Engineering of Jinan University,China) |
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| Abstract: | Anti-microbial photodynamic technology(APDT) on L.monocytogenes was conducted using photo sensitizer hematoporyrin monomethyl ether(HMME) followed by tungsten-halogen lamp(power density 200 mW/cm2).The bactericidal effects were measured by counting the reduction of colony forming unit(CFU) after APDT-HMME treatment.The degradation of protein was observed under sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE),and the damage of DNA was investigated by polymerase chain reaction(PCR).Approxi... |
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| Keywords: | hematoporyrin monomethyl ether anti-microbial photodynamic technology Listeria monocytogenes protein degradation DNA damage |
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