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| 黑木耳多糖的羧甲基化及其对肝癌细胞H ePG2的抑制作用 | |
| 引用本文： | 张华,王振宇,杨鑫,杨林,王雪. 黑木耳多糖的羧甲基化及其对肝癌细胞H ePG2的抑制作用[J]. 食品与机械, 2011, 27(3). DOI: 10.3969/j.issn.1003-5788.2011.03.013 |
| 作者姓名： | 张华 王振宇 杨鑫 杨林 王雪 |
| 作者单位： | 1. 哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨,150090 2. 哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨150090;东北林业大学,黑龙江哈尔滨150040 3. 德之馨(上海)有限公司,上海,201206 |
| 基金项目： | 国家高新技术研究发展计划(863计划) |
| 摘 要： | 对提取分离的黑木耳多糖进行羧甲基化,并对人体肝癌HepG2细胞株进行干扰研究,探讨其增殖抑制作用.将HepG2细胞进行贴壁培养,对处在对数生长期的HepG2细胞进行对照试验.结果表明,黑木耳中性多糖组、黑木耳酸性多糖组对HepG2细胞毒试验得到细胞存活率50%时的药物浓度范围在200～600 μg/mL.两种未经羧甲基化改性的黑木耳多糖在体外对人HepG2细胞具有抑制作用,而经羧甲基化改性的黑木耳多糖则没有. |
| 关 键 词： | 黑木耳酸性多糖 羧甲基化黑木耳酸性多糖 黑木耳中性多糖 羧甲基化黑木耳中性多糖 肝癌细胞HepG2 体外试验 |
In vitro antitumor activities of carboxymethylated derivatives of polysaccharides extracted from auricularia auricular |
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| ZHANG Hua,WANG Zhen-yu,YANG Xin,YANG Lin,WANG Xue. In vitro antitumor activities of carboxymethylated derivatives of polysaccharides extracted from auricularia auricular[J]. Food and Machinery, 2011, 27(3). DOI: 10.3969/j.issn.1003-5788.2011.03.013 | |
| Authors: | ZHANG Hua WANG Zhen-yu YANG Xin YANG Lin WANG Xue |
| Affiliation: | ZHANG Hua1 WANG Zhen-yu1,2 YANG Xin1 YANG Lin1 WANG Xue3 (1.Shool of Food Science and Engneering,Harbin Institute of Technology,Harbin,Heilongjiang 150090,China,2.Northeast Forestry University,Heilongjiang 150040,3.Symrise(Shanghai) Co.,Ltd.,Shanghai 201206,China) |
| Abstract: | Cancer has been a challenge for humanity.Many scientific researchers have been exploring antitumor activities of some natural products.It was reported that polysaccharide had good antitumor activities.This paper reports on a water-soluble polysaccharide(AAP) and in how it was ultrasound-assisted and extracted from Auricularia auricular and fractioned by Cetyl Trimethyl Ammonium Bromide(CTAB).Then these two carboxymethylated polysaccharides(acidic polysaccharide,AAAP and neutral polysaccharide,NAAP) were pre... |
| Keywords: | acid auricularia auricular polysaccharide(AAAP) carboxymethylated acid auricularia auricular polysaccharide(CMC AAAP) neutral auricularia auricula polysaccharide(NAAP) carboxymethylated neutral auricularia auricula polysaccharide(CMC NAAP) HepG2 in vitro |
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