| 酶法水解麦冬多糖及其产物的分离鉴定 |
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| 引用本文： | 吕宁,金凤燮,鱼红闪. 酶法水解麦冬多糖及其产物的分离鉴定[J]. 食品与发酵工业, 2011, 37(3) |
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| 作者姓名： | 吕宁 金凤燮 鱼红闪 |
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| 作者单位： | 大连工业大学生物工程学院,辽宁,大连,116034 |
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| 基金项目： | 国家自然科学基金，辽宁省高等学校优秀人才支持计划项目，辽宁省科学技术基金 |
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| 摘 要： | 采用生物酶法水解纯化后的麦冬多糖,并对其产物的成分进行分析。利用葡聚糖凝胶Sephadex G-100柱对麦冬粗多糖进行分离纯化,得到相对分子质量为48 347的纯麦冬多糖。使用Absidiasp.O8 s菌产酶水解纯麦冬多糖,产物经Sephadex G-100和Sephadex G-50柱分离,得到OP-1、OP-2、OP-3三种组分,相对分子质量分别为32 452、9 231和1 354。通过高效液相色谱分析纯麦冬多糖及其水解产物的单糖组成,结果表明,纯麦冬多糖的单糖组成为葡萄糖和果糖,其水解产物OP-1、OP-2、OP-3三种组分的单糖组成分别为果糖、果糖、葡糖糖和果糖。
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| 关 键 词： | 麦冬多糖 酶法水解 相对分子质量 高效液相色谱 |
Enzymatic Hydrolysis of Ophiopogon japonicus Polysaccharide and Isolation,Identification of the Products |
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| Lv Ning,Jin Feng-xie,Yu Hong-shan. Enzymatic Hydrolysis of Ophiopogon japonicus Polysaccharide and Isolation,Identification of the Products[J]. Food and Fermentation Industries, 2011, 37(3) |
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| Authors: | Lv Ning Jin Feng-xie Yu Hong-shan |
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| Affiliation: | Lv Ning,Jin Feng-xie,Yu Hong-shan(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China) |
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| Abstract: | The Ophiopogon japonicus polysaccharide was hydrolyzed by bio-enzyme and the products were analyzed.The relative molecular weight of the Ophiopogon japonicus polysaccharide was 48347,which was purified by Sephadex G-100.The Ophiopogon japonicus polysaccharide was hydrolyzed by enzyme of Absidia sp.O8s,and the products were then isolated using Sephadex G-100 and Sephadex G-50 to generate three components: OP-1,OP-2 and OP-3.Their relative molecular weights were 32452,9231,1354,respectively.From HPLC,the puri... |
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| Keywords: | Ophiopogon japonicus polysaccharide enzymatic hydrolysis relative molecular weight HPLC |
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