| 冬虫夏草原生质体诱变育种研究 |
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| 引用本文： | 朱蕴兰,陈安徽,王陶,陈宏伟.冬虫夏草原生质体诱变育种研究[J].食品科学,2010,31(5):256. |
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| 作者姓名： | 朱蕴兰 陈安徽 王陶 陈宏伟 |
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| 作者单位： | 徐州工程学院食品(生物)工程学院; |
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| 基金项目： | 江苏省高校自然科学研究计划项目(05KJD180203); 江苏省“333高层次人才培养工程”资助项目(2007.4) |
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| 摘 要： | 以冬虫夏草原生质体为材料,经紫外诱变获得了性状优于出发菌株的突变株。实验结果表明:生物量最高的再生菌株为诱变30s的8号菌株,生物量达到1.3445g/100mL,比出发菌株生物量提高55.16%;胞内多糖产量最高的再生菌株为诱变30s的3号菌株,胞内多糖产量达到54.570mg/g,比出发菌株多糖产量提高52.9%;富硒能力较高的突变菌株是诱变30s的8号菌株,富硒量达到125.8μg/g,比出发菌株提高50.11%。由结果可知,原生质体诱变技术可以用于冬虫夏草的菌种选育。
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| 关 键 词： | 冬虫夏草 原生质体 诱变 菌种选育 |
Ultraviolet-induced Protoplast Mutagenesis for Breeding High-performance Strains of Cordyceps sinensis |
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| ZHU Yun-lan,CHEN An-hui,WANG Tao,CHEN Hong-wei Engineering,Xuzhou Institute of Technology,Xuzhou ,China.Ultraviolet-induced Protoplast Mutagenesis for Breeding High-performance Strains of Cordyceps sinensis[J].Food Science,2010,31(5):256. |
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| Authors: | ZHU Yun-lan CHEN An-hui WANG Tao CHEN Hong-wei Engineering Xuzhou Institute of Technology Xuzhou China |
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| Affiliation: | ZHU Yun-lan,CHEN An-hui,WANG Tao,CHEN Hong-wei (College of Food(Biological) Engineering,Xuzhou Institute of Technology,Xuzhou 221008,China) |
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| Abstract: | Cordyceps sinensis was used as original strain to conduct protoplast mutagenesis with ultraviolet (UV) illumination for obtaining high-performance mutants. Results showed that 30-second UV illumination gave protoplast-regenerated strains of Cordyceps sinensis No.3 with the highest intercellular polysaccharide production and No.8 with the highest biomass and ability to enrich selenium. The intercellular polysaccharide content (calculated on the basis of dry weight of Cordyceps sinensis mycelia) in strain No.... |
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| Keywords: | Cordyceps sinensis protoplast mutagenesis strain breeding |
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