表皮葡萄球菌与血管内皮细胞的黏附作用及其机制

目的探讨表皮葡萄球菌与人脐静脉血管内皮细胞ECV304的黏附作用及其机制。方法将表皮葡萄球菌临床分离株通过刚果红琼脂检测胞间多糖黏附素(PIA);半定量生物被膜形成试验检测生物被膜表型;并在体外与ECV304细胞共同作用,在倒置显微镜下观察表皮葡萄球菌对ECV304细胞的黏附数。结果表皮葡萄球菌黏附ECV304细胞的数量随作用时间的延长而增加;PIA+/生物被膜表型+菌株与ECV304细胞的黏附数多于PIA+/生物被膜表型-菌株和PIA-/生物被膜表型-菌株,且差异有统计学意义,而PIA+/生物被膜表型-菌株和PIA-/生物被膜表型-菌株与细胞的黏附数差异无统计学意义。结论表皮葡萄球菌与血管内皮细胞的黏附有时间依赖性,能形成生物被膜的表皮葡萄球菌更易与血管内皮细胞黏附。

Adhesion of Staphylococcus epidermidis to Vein Endothelial Cells

Objective To investigate the mechanism of adhesion of Staphylococcus epidermidis to vein endothelial cells ECV304.Methods The clinical isolates of Staphylococcus epidermidis were determined for polysacchatide intercellular adhesion(PIA)by Congo red agarose electrophoresis and for phenotype of biofilm by semi-quantitative biofilm formation test,then co-cultured with ECV304 cells in vitro.The cells adhered with Staphylococcus epidermidis were counted under inverted microscope.Results The number of ECV304 cells adhered with Staphylococcus epidermidis increased with the increasing time for co-culture.The number of cells adhered with PIA+ / biofilm+ isolates was significantly larger than that with PIA+ / biofilm-and PIA-/ biofilm-isolates.Howev-er,no significant difference was observed between the numbers of cells adhered with PIA+ / biofilm-and with PIA-/ biofilm-isolates.Conclusion The adhesion of Staphylococcus epidermidis to vein endothelial cells was time-dependent.Compared with other Staphy-lococcus epidermidis isolates,the ones forming biofilm were easy to be adhered to vein endothelial cells.