Extraction and detection of DNA from Bacillus anthracis spores and the vegetative cells within 1 min |
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Authors: | Aslan Kadir Previte Michael J R Zhang Yongxia Gallagher Theresa Baillie Les Geddes Chris D |
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Affiliation: | Institute of Fluorescence, Laboratory for Advanced Medical Plasmonics, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA. |
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Abstract: | The use of a combination of low-cost technologies to both extract and detect anthrax DNA from spores and vegetative cells in two steps within 1 min is described. In a cavity, microwave energy is highly focused using thin-film aluminum "bow-tie" structures, to extract DNA from whole spores within 20 s. The detection of the released DNA, from less than 1000 vegetative cells, without additional preprocessing steps is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. The new platform technology presented here is a highly attractive alternative method for DNA extraction and the fast detection of gram-positive bacteria and potentially other pathogenic species and cells as well. |
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