| 氨基酸中空纤维膜分配色谱模型 |
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| 引用本文： | 白姝,薛博,史清洪,孙彦.氨基酸中空纤维膜分配色谱模型[J].化工学报,2003,54(7):978-983. |
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| 作者姓名： | 白姝 薛博 史清洪 孙彦 |
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| 作者单位： | 天津大学化工学院生物化工系，天津 300072 |
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| 基金项目： | 国家自然科学基金资助项目 (No 2 98760 3 0 )~~ |
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| 摘 要： | 以色氨酸为例，考虑氨基酸解离和在中空纤维膜内扩散传质建立了氨基酸中空纤维膜分配色谱理论模型.该模型采用完全独立的模型参数，模拟结果与实验数据吻合较好，验证了模型的可靠性.理论计算表明流动相的pH值与局部氨基酸浓度密切相关；理论和实验结果表明，流动相离子强度对氨基酸的保留行为有很大影响，采用梯度洗脱的方式可以改善洗脱峰形，提高色谱柱的分离度.
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| 关 键 词： | 中空纤维膜 色谱 氨基酸 液液萃取 模型 |
| 文章编号： | 0438-1157(2003)07-0978-06 |
| 收稿时间： | 2001-10-15 |
| 修稿时间： | 2001年10月15 |
MODELING OF HOLLOW-FIBER MEMBRANE PARTITIONING CHROMATOGRAPHY OF AMINO ACID |
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| BAI Shu,XUE Bo,SHI Qinghong,SUN Yan.MODELING OF HOLLOW-FIBER MEMBRANE PARTITIONING CHROMATOGRAPHY OF AMINO ACID[J].Journal of Chemical Industry and Engineering(China),2003,54(7):978-983. |
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| Authors: | BAI Shu XUE Bo SHI Qinghong SUN Yan |
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| Abstract: | A theoretical model for hollow-fiber membrane partition chromatography of amino acid was established by taking into account the dissociation of amino acid and mass transfer in the stationary phase immobilized in the membrane. With all the parameters determined independently, the model simulated very well the experimental chromatograms of a model amino acid, L-tryptophan, indicating the applicability of the model. Theoretical analysis also revealed the dependence of mobile phase pH on local amino acid concentration. In addition, the experimental and simulation results showed that mobile phase ionic strength had significant effect on the retention and peak shape of amino acid. Using a gradient elution mode can improve the peak shape and increase the column efficiency. |
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| Keywords: | hollow-fiber chromatography amino acid liquid-liquid extraction model |
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