| 利用玉米秸秆水解液发酵生产2,3-丁二醇 |
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| 引用本文： | 司阳,夏黎明.利用玉米秸秆水解液发酵生产2,3-丁二醇[J].食品与发酵工业,2010(2). |
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| 作者姓名： | 司阳 夏黎明 |
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| 作者单位： | 浙江大学化学工程与生物工程学系; |
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| 摘 要： | 利用1株克雷伯氏菌(Klebsiella oxytocaZU-03)发酵玉米秸秆水解液生产2,3-丁二醇,使水解液中的己糖和戊糖都得到了有效利用。研究结果表明,当玉米秸秆水解液中Na2SO4浓度低于20 g/L时,对2,3-丁二醇的发酵无明显影响。乙酸和乙醇是主要的发酵副产物,当浓度分别超过20 g/L和5 g/L时,对发酵的抑制作用较明显。玉米秸秆水解液发酵生产2,3-丁二醇的适宜初始pH值为6.0-6.5,初始总糖浓度为80-100 g/L。在总糖浓度80 g/L(含葡萄糖47.25 g/L,木糖32.75 g/L),初始pH值6.0,温度30℃的条件下发酵64 h,总糖利用率达到99.36%,2,3-丁二醇的质量浓度为37.20 g/L,产率为0.468 g/g(总糖),达到理论最大产率的94%。
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| 关 键 词： | 玉米秸秆 纤维素 半纤维素 酶水解 2 3-丁二醇 发酵 |
Fermentative Production of 2,3-butanediol from Corn Stover Hydrolysate |
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| Si Yang,Xia Liming.Fermentative Production of 2,3-butanediol from Corn Stover Hydrolysate[J].Food and Fermentation Industries,2010(2). |
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| Authors: | Si Yang Xia Liming |
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| Affiliation: | Si Yang,Xia Liming(Department of Chemical Engineering , Bioengineering,Zhejiang University,Hangzhou 310027,China) |
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| Abstract: | 2,3-butanediol is a valuable chemical feedstock and liquid fuel.Biological production of 2,3-butanediol from low-cost cellulosic material is of both economic and social benefits.Fermentation of 2,3-butanediol from corn stover hydrolysate by Klebsiella oxytoca ZU-03 was studied,during which both hexose and pentose in the hydrolysate were fully used as substrate.The results show that sodium sulfate concentration under 20 g/L in corn stover hydrolysate has no significant effects on fermentation;acetic acid and... |
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| Keywords: | corn stover cellulose semicellulose enzymatic hydrolysis 2 3-butanediol fermentation |
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