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Attachment of Salmonella typhimurium to Poultry Skin as Related to Cell Viability |
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| Authors: | KY KIM HS LILLARD JF FRANK SE CRAVEN |
| Affiliation: | Authors Kim, Lillard, and Craven are affiliated with the Poultry Microbiological Safety Research Unit, USDA, ARS, R. B. Russell Agricultural Research Center, P.O. Box 5677, Athens, GA 30604. Author Kim is also affiliated with the Dept. of Food Science &Technology, Univ. of Georgia, Athens, GA 30602. Author Frank is with the Dept. of Food Science &Technology, Univ. of Georgia, Athens, GA. |
| Abstract: | 3H-labeled S. typhimurium cells inactivated by gamma-radiation (60Co) and by formaldehyde (5%) were used to determine if cell viability affected attachment to poultry skin. Both gamma-irradiated and formal-dehyde-treated cells attached like live cells. There was no difference in attachment between cells grown in a chemically defined medium (SCDM) or in brain heart infusion broth (BHI). Live and gamma-irradiated cells and BHI-grown and SCDM-grown cells had similar surface hydrophobicity. Live and gamma-irradiated cells had similar cell surface charges, whereas BHI and SCDM-grown cells had different surface charges. Differences in cell surface charge did not affect attachment rate. |
| Keywords: | Salmonella typhimurium poultry skin cell viability |