Adsorption and recognition of protein molecular imprinted calcium alginate/polyacrylamide hydrogel film with good regeneration performance and high toughness |
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| Affiliation: | 1. State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Tianjin Polytechnic University, Tianjin 300387, China;2. School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China;3. The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;1. Major in Polymer Science & Engineering, School of Applied Chemical Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 702-701, Republic of Korea;2. National Institute for Nanomaterials Technology(NINT), Pohang University of Science and Technology(POSTECH), 77 Cheongam-ro, Nam-gu, Pohang 790-784, Republic of Korea;3. Department of Cogno-Mechatronics Engineering, Pusan National University, Busandaehak-ro 63 beon-gil 2, Geumjeong-gu, Busan 609-735, Republic of Korea;4. Department of Polymer Science & Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 702-701, Republic of Korea;1. Research Institute on Membrane Technology, ITM-CNR, c/o University of Calabria, Via P. Bucci, Cubo 17/C, 87030 Rende (CS), Italy;2. Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Rende (CS), Italy;1. Faculty of Chemistry, Adam Mickiewicz University in Poznań, Umultowska 89b, 61-614 Poznań, Poland;2. Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, Święcickiego 4, 60-781 Poznań, Poland;3. Institute of Human Genetics, Polish Academy of Sciences, Strzeszyńska 32, 60-479 Poznań, Poland;1. Department of Chemistry, FEPS, University of Surrey, Guildford, United Kingdom;2. Department of Chemistry, Sri Venkateswara University, Tirupati, India |
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| Abstract: | Protein imprinted calcium alginate/polyacrylamide hydrogel film (CA/PAM MIP) with high toughness was prepared using bovine serum albumin (BSA) as template molecule, sodium alginate and acrylamide as functional monomers, N,N′-methylenebisacrylamide (MBAA) as the covalent cross-linker and CaCl2 as the ionic cross-linker via UV radiation-reduced polymerization. Factors affecting the adsorption capacity and imprinting efficiency of the BSA-imprinted CA/PAM hydrogel films were investigated, such as ratio of polyacrylamide/sodium alginate, film thickness, MBAA concentration and CaCl2 concentration. Results showed that the CA/PAM MIP exhibited an obvious improvement in terms of adsorption capacity for BSA compared with non-imprinted polymer (NIP). The adsorption capacity of MIP for BSA reached 22.49 mg/g, which was 2.7 times higher than NIP. The regeneration property of the BSA-imprinted CA/PAM hydrogel was distinctly improved and the imprinting efficiency of CA/PAM MIP maintained 77.95% of the initial value after five repetitions. Single and binary proteins rebinding indicated that the CA/PAM MIP exhibited good recognition performance. Cell culture experiments showed CA/PAM MIP was more suitable for cell culture than CA/PAM NIP. The residual sodium dodecyl sulfate (SDS) in the elution process leaded to the death of mouse fibroblast cells (L929) after 3 days. A moderate elution solution without residue eluent should be used to prepare MIP for cell culture. |
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| Keywords: | Protein molecular imprinting Calcium alginate/polyacrylamide Hydrogel film Adsorption Recognition Cell culture |
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