Antibacterial activity of chitosan grafting nisin: Preparation and characterization |
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| Affiliation: | 1. Hubei Collaboration Innovation Center of Non-power Nuclear Technology, Hubei University of Science and Technology, Xianning 437100, China;2. College of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China;3. School of Nuclear Technology & Chemistry and Biology, Hubei University of Science and Technology, Xianning 437100, China;1. Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, 1177, Urmia, West Azarbaijan, Iran;2. Department of Analytical Chemistry, Faculty of Chemistry, Urmia University, 1177, Urmia, West Azarbaijan, Iran;3. Chemo and Biosensors Group, Faculty of Pharmacy, University of Jember, Jl. Kalimantan 37, Jember, 68121, Indonesia;1. School of Chemistry and Chemical Technology, Hefei University of Technology, Hefei 230009, Anhui, People''s Republic of China;2. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, Anhui, People''s Republic of China;3. Anhui Institute of Agro-Products Intensive Processing Technology, Hefei 230009, Anhui, People''s Republic of China;1. Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania;2. Department of Polymer Chemistry, Vilnius University, Vilnius, Lithuania;3. Laboratory of Genetics, Institute of Botany, Nature Research Centre, Vilnius, Lithuania;1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;2. Hunan Academy of Forestry, Changsha 410007, China;1. Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius, Lithuania;2. Laboratory of Genetics, Institute of Botany, Nature Research Centre, Vilnius, Lithuania;3. Department of Polymer Chemistry, Vilnius University, Vilnius, Lithuania |
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| Abstract: | Nisin grafted chitosan was prepared by using microbial transglutaminase as biocatalyst. The transglutaminase-catalyzed reaction displayed high efficiency, high selectivity, mild reaction condition and environmental friendliness. The results revealed that the degree of substitution (DS) of nisin–chitosan could be controlled by adjusting the reaction time, the reaction temperature and the molar ratio of nisin to chitosan. And nisin–chitosan in different pH showed excellent solubility. In addition, in vitro antibacterial activity assessment, nisin–chitosan with the concentration of 0.008 mg/mL showed pronounced inhibitory effect against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli). Furthermore, L929 mouse fibroblasts were cultured with nisin–chitosan, and the methylthiazol tetrazolium (MTT) assay showed that nisin–chitosan with the concentration from 0.005 to 0.01 mg/mL displayed low toxicity. The results may contribute to finding the application of nisin–chitosan in pharmaceutical and food industry fields. |
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| Keywords: | Chitosan Nisin Microbial transglutaminase Antibacterial activity Low toxicity |
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