The effect of chitosan nanoparticles onto Lactobacillus cells |
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| Affiliation: | 1. Health care department, Tosama d.o.o., Production of Medical Supplies, Vir, Šaranovičeva cesta 35, 1230 Domžale, Slovenia;2. CR Competence AB, Chemical Centre, Box 124, 22100 Lund, Sweden;3. University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Microbiology, Schrottova 39/1, HR-10000 Zagreb, Croatia;4. Savatech d.o.o., Industrial Rubber Products and Tyres, Škofjeloška cesta 6, 4000 Kranj, Slovenia;5. Institute for the Engineering and Design of Materials, Laboratory for Characterization and Processing of Polymers, University of Maribor, Smetanova 17, 2000 Maribor, Slovenia;1. Polymer Synthesis and Analysis Laboratory, Department of Chemistry, Faculty of Science and Arts, Canakkale Onsekiz Mart University, 17020 Canakkale, Turkey;2. Department of Bioengineering & Materials Science Engineering, Canakkale Onsekiz Mart University, 17020 Canakkale, Turkey;3. Department of Materials Science & Engineering, Faculty of Engineering, Canakkale Onsekiz Mart University, 17020 Canakkale, Turkey;4. Department of Chemical Engineering, Faculty of Engineering, Canakkale Onsekiz Mart University, 17020 Canakkale, Turkey |
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| Abstract: | Chitosan (CS) and trimethyl chitosan (TMC) solutions, as well as nanoparticles synthesized by ionic gelation method are studied. Their characterization is focused on determining the charge and antimicrobial properties against common pathogenic microorganisms and Lactobacillus spp., usually found in resident microbiota of vaginal and gastrointestinal tract. Special emphasis is given to the evaluation of antimicrobial activity in relation to the available cationic charge and the presence of nano-sized chitosan in comparison with chitosan macromolecules. In order to investigate the chitosan's antimicrobial mode of action diffusion nuclear magnetic resonance (D-NMR) is used as a novel approach. This technique enables the monitoring of chitosan nanoparticles (CSNP) effects onto healthy Lactobacillus cells. D-NMR results indicate that CSNP interact with the membrane of Lactobacillus cells, causing the perturbation of the membrane wall and lead to the death of the cells, suggesting the mechanism of Lactobacillus inhibition caused by CSNP. Lactobacillus inhibition is also reflected in low minimal inhibitory concentration (MIC). |
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