PLGA/SF blend scaffolds modified with plasmid complexes for enhancing proliferation of endothelial cells |
| |
| Affiliation: | 1. School of Chemical Engineering and Technology, Collaborative Innovation Center of Chemical Science and Chemical Engineering (Tianjin), Tianjin University, Tianjin 300072, China;2. Tianjin University-Helmholtz-Zentrum Geesthacht, Joint Laboratory for Biomaterials and Regenerative Medicine, Tianjin 300072, China;3. Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China;4. Department of Physiology and Pathophysiology, Logistics University of Chinese People’s Armed Police Force, Tianjin 300162, China;1. Renal Unit, Department of Medicine, University Hospital of Verona, Italy;2. Division of Nephrology and Dialysis, Columbus-Gemelli Hospital, Catholic University School of Medicine, Rome, Italy;3. Section of Dermatology and Venereology, Verona, Italy;4. Section of Nephrology, Azienda Ospedaliera \"Spedali Civili\" Brescia, Brescia, Italy;5. Institute of Nephrology, Dialysis and Renal Transplantation, S Orsola University Hospital, Bologna, Italy;6. Section of Nephrology, AOUC Azienda Ospedaliero-Universitaria Careggi, Firenze, Italy;7. Renal, Dialysis and Transplant Unit, Department of Emergency and Transplantation, University of Bari, Bari, Italy;8. First Surgical Clinic, Kidney Transplantation Center, University Hospital of Verona, Verona, Italy;1. Key Laboratory of National Education Ministry for Electromagnetic Processing of Materials, P.O. Box 314, Northeastern University, Shenyang 110004, China;2. Institute of Thermal Engineering, School of Energy and Power Engineering, Dalian University of Technology, Dalian 116024, China;1. Institute for Neuroimaging and Informatics, University of Southern California, Los Angeles, CA 90032, USA;2. Multimodal Imaging Laboratory, University of California, San Diego, CA 92037, USA;3. Department of Radiology, University of California, San Diego, CA 92037, USA;4. Department of Neurosciences, University of California, San Diego, CA 92037, USA;1. Department of Physics, University of Otago, PO Box 56, Dunedin, New Zealand;2. Department of Food Science, University of Otago, PO Box 56, Dunedin, New Zealand;1. Department of Ultrasound, Beijing Friendship Hospital, Capital Medical University, 95 Yongan Road, Beijing 100050, China;2. Department of Ultrasound, the Southern Building, Chinese People''s Liberation Army General Hospital, 28 Fuxing Road, Beijing 100853, China;3. Department of Ultrasound, Chinese People''s Liberation Army General Hospital, 28 Fuxing Road, Beijing 100853, China;4. Department of Pathology, Chinese People''s Liberation Army General Hospital, 28 Fuxing Road, Beijing 100853, China;1. University of Salerno, Italy;2. University of the Basque Country, Spain;3. University of Bath, United Kingdom of Great Britain and Northern Ireland |
| |
| Abstract: | Biomimetic scaffolds have been investigated for vascular tissue engineering for many years. However, the design of an ideal biodegradable vascular scaffold is still in progress. The optimization of poly(lactide-co-glycolide)/silk fibroin (PLGA/SF) blend composition was performed to provide the designed scaffolds with adequate mechanical properties and favorable biocompatibility for the intended application. By systematically varying the weight ratio of PLGA and SF, we could control fiber diameter and hydrophilicity as well as mechanical properties of the fibrous scaffolds. These scaffolds with a weight ratio of PLGA/SF at 70/30 exhibited excellent performance, such as tensile strength of 1.5 ± 0.1 MPa, and elongation at break of 77.4 ± 6.4%. Therefore, PLGA/SF scaffold with a weight ratio of 70/30 was chose as the matrix because it matches at best the mechanical demands for application in vascular tissue engineering. In order to promote the endothelialization of electrospun scaffolds, we used pEGFP-ZNF580 plasmid (pZNF580) complexes to modify the electrospun scaffolds by electrospraying technique. pZNF580 complexes were prepared from pZNF580 and microparticles (MPs) of amphiphilic copolymer methoxy-poly(ethylene glycol)-block-poly(3(S)-methyl-2,5-morpholinedione-co-glycolide)-graft-polyethyleneimine. Negatively charged PLGA/SF fibers adsorbed the positively charged MPs via physical deposition and electrostatic force. Scanning electron microscope image indicated the forming of composite scaffold and MPs did not change fiber’s shape and 3-D structure. Cell culture experiments demonstrated that the scaffolds modified with MPs/pZNF580 complexes could promote human umbilical vein endothelial cell growth and inhibit human umbilical artery smooth muscle cell proliferation. Our results indicated that the composite scaffolds with MPs/pZNF580 complexes could be used as a potential scaffold for vascular tissue engineering. |
| |
| Keywords: | Electrospinning Electrospraying Microparticles Scaffolds Vascular grafts |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
