| 酶法制备n-3多不饱和脂肪酸型磷脂的工艺 |
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| 引用本文： | 孙兆敏,李金章,丛海花,王玉明,薛长湖. 酶法制备n-3多不饱和脂肪酸型磷脂的工艺[J]. 中国油脂, 2010, 35(4) |
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| 作者姓名： | 孙兆敏 李金章 丛海花 王玉明 薛长湖 |
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| 作者单位： | 中国海洋大学,食品科学与工程学院,山东,青岛,266003 |
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| 摘 要： | 研究了无溶剂体系中固定化磷脂酶A1催化大豆磷脂与乙酯型鱼油进行酯交换反应,制备n-3多不饱和脂肪酸型磷脂的工艺。考察了反应温度、反应时间、底物比(乙酯型鱼油与磷脂的质量比)以及加水量的影响,得到了较优的反应条件。实验结果表明,加酶量(按大豆磷脂的质量计算)20%,底物比8∶1,底物总质量5.0g,加水量55μL,55℃反应12h时,所得产物中EPA和DHA的含量分别为8.0%和17.8%。
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| 关 键 词： | 磷脂酶A1 二十碳五烯酸(EPA) 二十二碳六烯酸(DHA) 磷脂 |
Enzymatic synthesis of phospholipids enriched with n-3 polyunsaturated fatty acids |
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| SUN Zhaomin,LI Jinzhang,CONG Haihua,WANG Yuming,XUE Changhu. Enzymatic synthesis of phospholipids enriched with n-3 polyunsaturated fatty acids[J]. China Oils and Fats, 2010, 35(4) |
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| Authors: | SUN Zhaomin LI Jinzhang CONG Haihua WANG Yuming XUE Changhu |
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| Affiliation: | SUN Zhaomin,LI Jinzhang,CONG Haihua,WANG Yuming,XUE Changhu(College of Food Science , Engineering,Ocean University of China,Qingdao 266003,Sh,ong,China) |
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| Abstract: | Transesterification of soybean phospholipids and fish oil in the form of ethyl esters was catalyzed by immobilized phospholipase A1 in a solvent-free system to synthesize phospholipids enriched with n-3 polyunsaturated fatty acids. The effects of the factors such as temperature,reaction time,mass ratio of fish oil to soybean phospholipids and water dosage on the reaction were studied. The optimal conditions were as follows:20% immobilized phospholipase A1(basis of the phospholipids mass),mass ratio of fish ... |
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| Keywords: | phospholipase A1 eicosapentaenoic acid (EPA) docosahexaenoic acid (DHA) phospholipids |
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