海藻酸钠与羧甲基纤维素钠固定化高温碱性脂肪酶

以海藻酸钠和羧甲基纤维素钠为复合载体，研究包埋法固定化毕赤酵母高温碱性脂肪酶。通过单因素试验考察了不同载体、载体浓度、酶与载体配比等因子对脂肪酶固定化的影响，并采用正交试验对脂肪酶固定化条件进行了优化。结果表明，包埋法固定化脂肪酶的最优条件为海藻酸钠含量1.0%、羧甲基纤维素钠含量0.25%、加酶量50 U/(g载体)、CaCl2浓度0.4 mol/L，固定化时间40 min。在最优固定化条件下，固定化脂肪酶酶活收率达99.50%。

Immobilization of high-temperature alkaline lipase by sodium alginate and carboxymethyl cellulose sodium

Sodium alginate and carboxymethyl cellulose sodium were used as carriers to research the immobilization of high-temperature alkaline lipase from Pichia pastoris. Effects of different carriers, carrier concentration, enzyme-to-carrier ratio on the lipase immobilization were investigated by single factor experiments, and the optimum conditions for immobilization were determined by orthogonal experiment. The results indicated that the optimized conditions for immobilized lipase were sodium alginate 1.0%, carboxymethyl cellulose sodium 0.25%, enzyme addition 50 U/g, CaCl2 concentration 0.4 mol/L, and immobilization time 40 min. According to the optimum conditions, the recovery of the immobilized lipase activity reached 99.50%.