A gas chromatographic method for testing antioxidants |
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Authors: | Astri Rogstad Ragnhild Reinton |
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Affiliation: | (1) Department of Food Hygiene, Veterinary College of Norway, Oslo-Dep., P.O. Box 8146, Oslo 1, Norway |
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Abstract: | A gas chromatographic method for evaluating the effect of antioxidants is described. Emulsions of linoleic acid both with
and without antioxidant are oxidized enzymatically. Concentrations of uncreacted linoleic acid are measured at varying incubation
times and various concentrations of both lipoxidase and antioxidant. Two antioxidants have been tested. The method is simple,
precise, and reproducible. The inhibition mechanism and experimental conditions are discussed. |
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