| 高产植酸酶酵母Candida Krusei WZ-001的等离子束诱变选育 |
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| 引用本文： | 梁彦龙,王运吉,张苓花.高产植酸酶酵母Candida Krusei WZ-001的等离子束诱变选育[J].大连工业大学学报,2002,21(4). |
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| 作者姓名： | 梁彦龙 王运吉 张苓花 |
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| 摘 要： | 从土壤中筛选得到的 1株高产植酸酶酵母菌CandidaKruseiWZ 0 0 1 ,利用等离子诱变方法对这一菌株进行诱变 ,获得 1株植酸酶高产突变株 ,其酶活性比出发菌株提高了 98%。比较了N+、H+、Zn2 +3种离子注入菌体的诱变效果 ,实验结果表明N+离子注入效果最佳 ,注入最佳剂量为 5 0× 1 0 13N+/cm2 。
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| 关 键 词： | 克鲁斯假丝酵母 植酸酶 离子注入 |
Breeding of high-yield phytase yeast Candida Krusei WZ-001 by ion implantation |
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| Abstract: | Screening from soil, we find a high-yield phytase yeast identified as Candida Krusei WZ-001. By mutagenized ion implantating, we breed the strain with phytase activity elevated to 98% of that of the original strain. The mutagenic effects of N + implantation, H + implantation, and Zn 2+ implantation are compared with each other. It indicates that the mutagenic effect of N + implantation is the best with an optimal implanting dose 50×10 13 N +/cm 2. |
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| Keywords: | Candida Krusei phytase ion implantation |
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