| 瑶山甜茶可溶性糖的测定 |
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| 引用本文： | 邹青松,陈山,王晓,陈玮,李素霞,韦艳君. 瑶山甜茶可溶性糖的测定[J]. 食品工业科技, 2011, 0(1): 296-299 |
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| 作者姓名： | 邹青松 陈山 王晓 陈玮 李素霞 韦艳君 |
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| 作者单位： | 广西大学糖业工程技术研究中心,广西,南宁,530004 |
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| 基金项目： | 广西区自然科学基金项目(0731051); 广西区研究生创新项目(105930903060) |
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| 摘 要： | 为探明瑶山甜茶中可溶性糖的含量和种类,采用蒽酮—硫酸比色法测定其可溶性总糖含量,运用高效凝胶过滤色谱法(HPGFC)测定多糖分子量分布范围,并利用高效液相色谱—蒸发光散射检测器(HPLC-ELSD)法在两种流动相体系中测定和分析其低分子寡糖和单糖的种类与含量。结果表明,瑶山甜茶中可溶性总糖含量为15.20%,相对标准偏差(RSD)为0.996%;多糖分子量Mw范围为51551～55628,RSD为0.64%～1.23%;单糖种类主要为木糖、果糖和葡萄糖,相对含量分别是0.54%、52.18%和42.76%。
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| 关 键 词： | 瑶山甜茶 可溶性糖 蒽酮-硫酸法 高效凝胶过滤色谱法(HPGFC) 高效液相-蒸发光散射法(HPLC-ELSD) |
Determination of the soluble sugar in Rubus Suavissimus S.Lee |
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| ZOU Qing-song,CHEN Shan,WANG Xiao,CHEN Wei,LI Su-xia,WEI Yan-jun. Determination of the soluble sugar in Rubus Suavissimus S.Lee[J]. Science and Technology of Food Industry, 2011, 0(1): 296-299 |
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| Authors: | ZOU Qing-song CHEN Shan WANG Xiao CHEN Wei LI Su-xia WEI Yan-jun |
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| Affiliation: | ZOU Qing-song,CHEN Shan,WANG Xiao,CHEN Wei,LI Su-xia,WEI Yan-jun(Center for Sugar Technology Research,Guangxi University,Nanning 530004,China) |
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| Abstract: | To investigate the content and component of the soluble sugar in R.Suavissimus S.Lee,an anthrone-sulfuric acid chromatometry method was used to determine total soluble sugar content,and a high performance filter chromatography(HPGFC)was used to determine the molecular of its polysaccharide,and so was high performance liquid chromatography-evaporative light scattering detection(HPLC-ELSD)to determine the component and quantity of the monosaccharide and low-molecular-oligosaccharide. The results showed that t... |
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| Keywords: | Rubus Suavissimus S.Lee soluble sugar anthrone-sulfuric acid chromatometry HPGFC HPLC-ELSD |
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