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分光光度法测定食品色素含量的研究
引用本文:杜建中,李青梅,卢冬梅,朱艺强,丁玎,罗科丽,谭永辉,赖春莲. 分光光度法测定食品色素含量的研究[J]. 食品工业科技, 2011, 0(1): 300-303
作者姓名:杜建中  李青梅  卢冬梅  朱艺强  丁玎  罗科丽  谭永辉  赖春莲
作者单位:1. 湛江师范学院,广东高校新材料工程技术开发中心,广东,湛江,524048
2. 圣地亚哥州立大学公共卫生学院,美国圣地亚哥92123;加州大学圣地亚哥分校预防医学系,美国圣地亚哥92037
基金项目:湛江师范学院科学研究基金资助项目(L0514)
摘    要:采用单波长法对饮料中胭脂红和亮蓝含量进行测定,结果显示,胭脂红在0.2~80mg/L,亮蓝在0.1~20mg/L浓度范围具有良好线性关系,回收率在98%~108%。采用单波长法和双波长法相结合对饮料中酒石黄和日落黄含量进行测定,结果显示,酒石黄在0.1~70mg/L,日落黄在0.2~60mg/L浓度范围具有良好线性关系,回收率在95%~110%。两种方法对样品检测快速、准确,且操作简便。利用双波长比值光谱法对果味汽水中酒石黄、日落黄、胭脂红含量进行测定,结果显示,酒石黄在0.1~60mg/L,日落黄在0.8~90mg/L,胭脂红在0.2~80mg/L浓度范围内具有良好的线性关系,平均回收率分别为100.5%、102.4%和101.8%。

关 键 词:双波长法  双波长比值法  胭脂红  亮蓝  酒石黄  日落黄

Study on determination of pigments in food by single wavelength spectrophotometer and dual wavelength spectrophotometer
DU Jian-zhong,LI Qing-mei,LU Dong-mei,ZHU Yi-qiang,DING Ding,LUO Ke-li,TAN Yong-hui,LAI Chun-lian. Study on determination of pigments in food by single wavelength spectrophotometer and dual wavelength spectrophotometer[J]. Science and Technology of Food Industry, 2011, 0(1): 300-303
Authors:DU Jian-zhong  LI Qing-mei  LU Dong-mei  ZHU Yi-qiang  DING Ding  LUO Ke-li  TAN Yong-hui  LAI Chun-lian
Affiliation:DU Jian-zhong1,LI Qing-mei1,LU Dong-mei1,ZHU Yi-qiang1,DING Ding2,3,LUO Ke-li1,TAN Yong-hui1,LAI Chun-lian1(1.Development Center for New Materials Engineering and Technology in Universities of Guangdong,Zhanjiang Normal University,Zhanjiang 524048,China,2.Graduate School of Public Health,San Diego State University,San Diego 92123,USA,3.Department of Family and Preventive Medicine,University of California,San Diego,San Diego 92037,USA)
Abstract:The single wavelength spectrophotometer was used to determine both ponceaue and brilliant blue of beverage. The results showed a good linear relationship for ponceaue at 0.2 ~ 80mg/L and for brilliant blue at 0.1~20mg/L,the recovery was between 98% and 108%. Both single wavelength and dual wavelength spectrophotometer were used to determine tartrazine and sunset yellow in beverage simultaneously. The results showed a good linear relationship for the tartrazine at 0.1~70mg/L and sunset yellow at 0.2 ~ 60mg/L...
Keywords:dual wavelength spectrophotometer  ponceaue  brilliant blue  tartrazine  sunset yellow  
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