| 食源性致病菌PCR检测中常用的靶基因及其参考引物 |
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| 引用本文： | 宋艳,李建林,郑铁松. 食源性致病菌PCR检测中常用的靶基因及其参考引物[J]. 食品工业科技, 2011, 0(1): 371-376 |
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| 作者姓名： | 宋艳 李建林 郑铁松 |
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| 作者单位： | 南京师范大学金陵女子学院,江苏,南京,210097 |
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| 摘 要： | PCR方法检测食源性致病菌在敏感性、特异性与速度上都具有很大优势,在其应用过程中,选择的靶基因和由此设计的引物序列直接影响方法的特异性和灵敏性。综述了大肠杆菌、沙门氏菌、金黄色葡萄球菌、志贺氏菌及其他常见的食源性致病菌在PCR检测中常用的靶基因及其参考引物,以供相关研究者借鉴。
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| 关 键 词： | PCR 食源性致病菌 靶基因 引物 |
Molecular targets and primers-associated for the PCR detection of foodborne pathogen |
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| SONG Yan,LI Jian-lin,MENG Tie-song. Molecular targets and primers-associated for the PCR detection of foodborne pathogen[J]. Science and Technology of Food Industry, 2011, 0(1): 371-376 |
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| Authors: | SONG Yan LI Jian-lin MENG Tie-song |
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| Affiliation: | SONG Yan,LI Jian-lin,ZHENG Tie-song(Jinling College,Nanjing Normal University,Nanjing 210097,China) |
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| Abstract: | The PCR detection of foodborne pathogen is rapid,accurate,specific and sensitive. Molecular targets and primers-associated could effect the specification and sensitivity of PCR detection directly. The paper reviewed the studies on the molecular targets and primers-associated for the PCR detection of Escherichia coli,Salmonella,Staphylococcus aureus,Shigella and other foodborne pathogen,which would be a great pool of information resources to select specific targets for the PCR detection. |
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| Keywords: | PCR foodborne pathogen molecular target primers |
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