| 桑黄菌丝体多糖的分离纯化及理化性质分析 |
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| 引用本文： | 谢丽源,张勇,郭勇,吴翔,彭卫红,甘炳成. 桑黄菌丝体多糖的分离纯化及理化性质分析[J]. 食品科学, 2011, 32(5): 143-147. DOI: 10.7506/spkx1002-6630-201105032 |
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| 作者姓名： | 谢丽源 张勇 郭勇 吴翔 彭卫红 甘炳成 |
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| 作者单位： | 1.四川省农业科学院土壤肥料研究所 2.电子科技大学生命科学与技术学院 |
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| 基金项目： | 四川省青年基金项目(2008ZQ026-072); 四川省科技支撑计划项目(2008FZ0157) |
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| 摘 要： | 研究桑黄粗多糖的分离和纯化工艺，并对多糖组分进行理化性质分析。结果表明：经DEAE-52纤维素离子交换层析和Sephadex G-100凝胶过滤层析得到两个组分P-47000和P-8700；经高效液相色谱分析证明，两组分均为纯品，且不含蛋白质、核酸，为非淀粉类多糖，分子质量分别为4.74×104D和8.71×103D，均由鼠李糖、阿拉伯糖、木糖、葡萄糖、半乳糖组成，P-47000中各单糖物质的量比为3.47:1.99:1:63.27:13.44，P-8700中各单糖物质的量比为10.46:1:1.03:182.75:30.94。
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| 关 键 词： | 桑黄 多糖 纯化 理化性质 |
| 收稿时间： | 2010-06-19 |
Isolation, Purification and Physico-chemical Characteristics Analysis of Mycelial Polysaccharides from Phellinus baumii |
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| XIE Li-yuan,ZHANG Yong,,GUO Yong,WU Xiang,PENG Wei-hong,GAN Bing-cheng. Isolation, Purification and Physico-chemical Characteristics Analysis of Mycelial Polysaccharides from Phellinus baumii[J]. Food Science, 2011, 32(5): 143-147. DOI: 10.7506/spkx1002-6630-201105032 |
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| Authors: | XIE Li-yuan ZHANG Yong GUO Yong WU Xiang PENG Wei-hong GAN Bing-cheng |
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| Affiliation: | 1. Institute of Soil and Fertilizer, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China；2. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China |
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| Abstract: | The isolation,purification and physi-cochemical characterization of mycelial polysaccharides from Phellinus baumii were studied.Two polysaccharide fractions were separated from crude deproteinized polysaccharides from Phellinus baumii mycelia by DEAE-52 cellulose column chromatography,followed by purification on Sephadex G-100 gel filtration column.The two fractions were determined by HPLC to contain neither protein nor nuclear acid and to be pure non-starch polysaccharides,their molecular weights were 4.74... |
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| Keywords: | Phellinus baumii polysaccharide purification physico-chemical characteristics |
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