饮料和糖果中5种非法添加色素的检测

采用聚酰胺吸附法提取样品溶液的色素，用乙醇-氨水-水混合溶液解吸，用高效液相色谱-二极管阵列检测器测定。采用ODS柱分离，以甲醇和硫酸铵溶液为流动相进行洗脱，在520nm波长处检测。亮黑、荧光素钠、红色2G、荧光桃红和孟加拉玫瑰红的质量浓度在1.0～10.0mg/L范围内与其色谱峰面积的线性关系良好(r=0.999)，在1、2、5mg/kg添加水平时的平均回收率在82.95%～95.43%范围内，RSD值在1.99%～5.95%范围内。方法检出限为1.0mg/kg。该方法准确度高，分离效能好，结果稳定可靠，适合于饮料和糖果中亮黑、荧光素钠、红色2G、荧光桃红和孟加拉玫瑰红的测定。

Determination of 5 Kinds of Illegally-added Colorants in Drink and Candy

A quantitative determination method for Brilliant Black PN, Fluorescein Sodium, Red 2G, Phloxine B and Rose Bengal in drink and candy was established using high performance liquid chromatography (HPLC). For sample preparation, extraction by polyamide adsorption and desorption with ethanol-ammonia solution-water (7:2:1, V/V) were carried out. An ODS C18 column (250 mm × 4.6 mm, 5μm) was used for chromatographic separation, which was eluted with methanol-0.02 mol/L ammonium acetate aqueous solution as mobile phase at 35 ℃. The linear range of the developed method was 1.0－10.0 mg/L with relative standard deviation of 1.99%－5.95% and the detection limit was 1.0 mg/kg. The average recovery rates were 82.95%－95.43%. This method is simple, accurate, and suitable for the determination of these illegally-added colorants in drink and candy.