| 3株罗伦隐球酵母的26S rDNAD1/D2区序列、代谢指纹图谱及生防效力比较分析 |
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| 引用本文： | 姚子鹏,郭晓敏,王友升,李丽萍. 3株罗伦隐球酵母的26S rDNAD1/D2区序列、代谢指纹图谱及生防效力比较分析[J]. 食品科学, 2011, 0(1) |
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| 作者姓名： | 姚子鹏 郭晓敏 王友升 李丽萍 |
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| 作者单位： | 北京工商大学植物资源研究开发北京市重点实验室;北京工商大学食品添加剂与配料北京高校工程研究中心; |
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| 基金项目： | 国家自然科学基金项目(30901009); 北京市科技新星项目(2007B011) |
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| 摘 要： | 研究3株不同来源的酵母拮抗菌(Crytococcus laurentii)001#、010#和012#的种间亲缘关系、Biolog微生物鉴定系统中的不同碳源物质代谢指纹图谱以及对于不同果蔬的侵染病原菌的生防效力。结果表明:亲缘关系相近的菌株001#和010#间的碳源代谢指纹图谱中存在43种相同的最适碳源和13种相同的可利用碳源,菌株012#则与001#和010#利用碳源情况差异性较大,但是它们分别在梨、油桃和芦笋果实上的生防效力却有一些差别,这可能与不同菌株所特异利用的碳源种类以及果蔬中含有的特异的碳源种类和含量有关。发现不同果实的碳源成分和比例引起的拮抗菌生防效果差异的现象,并揭示亲缘关系能在一定程度上通过菌株的碳源代谢特征表现出来,为酵母拮抗菌在生防效力作用机理研究提供参考。
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| 关 键 词： | 酵母拮抗菌 分子生物学聚类分析 代谢指纹图谱 生防效力 |
Comparative Analyses of 26S rDNAD1/D2 Sequence,Metabolic Fingerprint and Bio-defense Capability of Three Strains of Cryptococcus laurentii |
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| YAO Zi-peng,GUO Xiao-min,WANG You-sheng,LI Li-ping. Comparative Analyses of 26S rDNAD1/D2 Sequence,Metabolic Fingerprint and Bio-defense Capability of Three Strains of Cryptococcus laurentii[J]. Food Science, 2011, 0(1) |
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| Authors: | YAO Zi-peng GUO Xiao-min WANG You-sheng LI Li-ping |
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| Affiliation: | YAO Zi-peng1,2,GUO Xiao-min1,WANG You-sheng1,LI Li-ping1,2(1.Beijing Key Laboratory of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048,China,2.Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients,China) |
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| Abstract: | Three different strains(numbered as 001#,010# and 012#) of antagonistic yeast Crytococcus laurentii were investigated to understand their phylogenetic relationships and metabolic fingerprints in Biolog microbial identification system and biodefense efficacies against the main pathogens of fruits and vegetables.The results indicated that strains 001# and 010# had a close phylogenetic relationship and there were 43 optimal and 13 utilizable carbon sources in both of their carbon source metabolic fingerprints.... |
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| Keywords: | antagonistic yeast cluster analysis metabolic fingerprint biodefense capability |
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