| 冻干鲤鱼粉中恩诺沙星和环丙沙星残留测定的两种前处理方法比较 |
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| 引用本文： | 赵艳,王丽敏,刘耀敏,张凤枰. 冻干鲤鱼粉中恩诺沙星和环丙沙星残留测定的两种前处理方法比较[J]. 食品科学, 2011, 0(2) |
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| 作者姓名： | 赵艳 王丽敏 刘耀敏 张凤枰 |
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| 作者单位： | 通威股份有限公司;中国科学院西北高原生物研究所;中国科学院研究生院; |
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| 摘 要： | 采用固相萃取和液液萃取结合超高效液相色谱-串联质谱法(UPLC-MS/MS),对冻干鲤鱼粉中恩诺沙星和环丙沙星残留量进行测定,比较两种前处理方法的测定结果。固相萃取法:样品经EDTA-Mcllvaine缓冲溶液提取,HLB固相萃取柱净化,氮气浓缩;液液萃取法:样品经酸性乙腈溶液提取,正己烷液液萃取净化,旋转蒸发浓缩。两种方法的提取液浓缩定容后,以超高效液相色谱-串联质谱仪测定,外标法定量。固相萃取和液液萃取法的回收率分别为95%～112%和75%～90%,RSD分别为2.9%～7.4%和1.0%～4.6%,检出限分别为1.0μg/kg和0.1～0.2μg/kg。结果表明,两种方法简便快速、重现性好、选择性强,均适用于鱼肉和冻干鲤鱼粉中恩诺沙星和环丙沙星残留的测定。
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| 关 键 词： | 恩诺沙星 环丙沙星 残留 冻干鲤鱼粉 超高效液相色谱-串联质谱法(UPLC-MS/MS) |
Comparison of Two Pretreatment Methods for UPLC-MS/MS Determination of Enrofloxacin and Ciprofloxacin Residues in Freeze-dried Carp Meal |
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| ZHAO Yan,WANG Li-min,LIU Yao-min,ZHANG Feng-ping,. Comparison of Two Pretreatment Methods for UPLC-MS/MS Determination of Enrofloxacin and Ciprofloxacin Residues in Freeze-dried Carp Meal[J]. Food Science, 2011, 0(2) |
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| Authors: | ZHAO Yan WANG Li-min LIU Yao-min ZHANG Feng-ping |
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| Affiliation: | ZHAO Yan1,WANG Li-min1,LIU Yao-min1,ZHANG Feng-ping1,2,3,*(1.Tongwei Co.Ltd.,Chengdu 610041,China,2.Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,3.Graduate University of Chinese Academy of Sciences,Beijing 100049,China) |
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| Abstract: | Sample pretreatment was achieved by two different methods,solid-phase extraction and liquid-liquid extraction,before the analysis of enrofloxacin and ciprofloxacin residues in freeze-dried carp meal on UPLC-MS/MS.The analytical results were compared to examine if the two pretreatment methods influence the accuracy of determination.The solid-phase extraction procedure was composed of extraction with EDTA-Mcllvaine buffer,clean-up on HLB column and blowing to dryness under nitrogen gas.Liquid-liquid extractio... |
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| Keywords: | enrofloxacin ciprofloxacin residues freeze-dried carp meal UPLC-MS/MS |
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