| 杜仲粕中桃叶珊瑚苷的分离和纯化 |
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| 引用本文： | 李辉,文赤夫,李亚男,彭玉丹,汪兰,张永康.杜仲粕中桃叶珊瑚苷的分离和纯化[J].食品科学,2011,32(2):32-35. |
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| 作者姓名： | 李辉 文赤夫 李亚男 彭玉丹 汪兰 张永康 |
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| 作者单位： | 1. 吉首大学生物资源与环境科学学院 2. 吉首大学 植物资源保护与利用省级重点实验 |
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| 基金项目： | 湖南省教育厅优秀青年项目(06B076) |
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| 摘 要： | 为研究大孔树脂对桃叶珊瑚苷的吸附与解吸性能及分离纯化效果，通过静态吸附与解吸实验从12 种大孔树脂中筛选高性能树脂，用树脂进行柱层析分离目标产物并优化分离条件。结果表明：大孔树脂SIPI-7、HZ-820和S-8 对目标分子既有高的吸附率，又能有效脱附；当用HZ-820 型树脂进行柱层析分离纯化桃叶珊瑚苷时，优化分离条件为上样量0.5g/g(以生药质量:树脂质量计)、乙醇- 水- 冰醋酸混合溶剂(50:50:3，V/V)为洗脱剂、流速1.0mL/min。在优化条件下，收集含产物浓度高的流分，经浓缩和重结晶后所获产品中桃叶珊瑚苷纯度高达96.82%，且具有可观的产品得率。
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| 关 键 词： | 桃叶珊瑚苷 分离纯化 大孔树脂 杜仲粕 |
| 收稿时间： | 2010-04-02 |
Separation and Purification of Aucubin from Eucommia ulmoides Seed Meal |
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| LI Hui,WEN Chi-fu,LI Ya-nan,PENG Yu-dan,WANG Lan,ZHANG Yong-kang.Separation and Purification of Aucubin from Eucommia ulmoides Seed Meal[J].Food Science,2011,32(2):32-35. |
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| Authors: | LI Hui WEN Chi-fu LI Ya-nan PENG Yu-dan WANG Lan ZHANG Yong-kang |
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| Affiliation: | 1. College of Biology and Environmental Science, Jishou University, Jishou 416000, China；
2. Hunan Provincial Key Laboratory of Plant Resource Protection and Exploitation, Jishou University, Jishou 416000, China |
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| Abstract: | The objective of this study was to develop a macroporous resin adsorption method for separating and purifying aucubin in Eucommia ulmoides seed meal extracted into 80%aqueous ethanol solution.Twelve kinds of macroporous resins were compared for differences in their adsorption and desorption performances towards aucubin,and the results indicated that macroporous resins SIPI-7,HZ-820 and S-8 had better abilities to adsorb and desorb aucubin,among which,HZ-820 was considered to be the best resin due to the hig... |
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| Keywords: | aucubin separation and purification macroporous resin Eucommia ulmoides seeds |
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