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| 转基因玉米的多重PCR－毛细管电泳紫外检测技术研究 | |
| 引用本文： | 张春娇,许文涛,程国灵,赵维薇,戴蕴青,罗云波,黄昆仑.转基因玉米的多重PCR－毛细管电泳紫外检测技术研究[J].食品工业科技,2011,32(2):328-333. |
| 作者姓名： | 张春娇 许文涛 程国灵 赵维薇 戴蕴青 罗云波 黄昆仑 |
| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083 2. 中国农业大学食品科学与营养工程学院,北京,100083;农业部转基因生物食用安全检验监督测试中心,北京,100083 3. 农业部转基因生物食用安全检验监督测试中心,北京,100083 |
| 基金项目： | 国家高技术研究发展计划(863)项目(2006AA10Z440); 国家自然基金(30800770); 转基因生物重大专项(2008ZX08012-001) |
| 摘 要： | 建立了转基因玉米Ly038、Mon863和Mon810的品系特异性基因多重PCR产物的毛细管电泳-紫外检测方法。根据三种转基因玉米的基因序列设计多重PCR引物,优化PCR扩增体系和条件,以8.0g/L羟乙基纤维素为筛分介质,用毛细管电泳-紫外检测法同时检测出三种玉米的品系特异性基因,11.195min内即可完成检测。用Origin软件对电泳结果进行分析并得出不同范围的DNA曲线方程,样品出峰时间的相对误差在1.03%～5.02%之间。并对纯化前后的样品进行了毛细管电泳分离的对照,发现PCR产物纯化后更适于紫外检测的毛细管电泳。多重PCR具有节约模板、节省试剂和缩短检测时间的优点,毛细管相对于传统的琼脂糖凝胶电泳,具有高效、快速、灵敏且经济环保的优点,所以此方法可广泛地应用于食品安全检测和临床检验等领域中。 |
| 关 键 词： | 转基因玉米 多重PCR 毛细管电泳 紫外检测 |
Study on genetically modified maize by multiplex polymerase chain reaction-capillary electrophoresis with UV detection |
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| ZHANG Chun-jiao,XU Wen-tao,CHENG Guo-ling,ZHAO Wei-wei,DAI Yun-qing,LUO Yun-bo,HUANG Kun-lun.Study on genetically modified maize by multiplex polymerase chain reaction-capillary electrophoresis with UV detection[J].Science and Technology of Food Industry,2011,32(2):328-333. | |
| Authors: | ZHANG Chun-jiao XU Wen-tao CHENG Guo-ling ZHAO Wei-wei DAI Yun-qing LUO Yun-bo HUANG Kun-lun |
| Affiliation: | ZHANG Chun-jiao1,XU Wen-tao1,2,CHENG Guo-ling1,ZHAO Wei-wei1,DAI Yun-qing2,LUO Yun-bo1,HUANG Kun-lun1,2 (1..College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China,2.The Supervision,Inspection & Testing Center of Genetically Modified Orginisms Food Safety,Ministry of Agriculture,China) |
| Abstract: | Event-specific qualitative detection of three kinds of genetically modified(GM)maize by multiplex polymerase chain reaction-capillary electrophoresis(CE)with UV detection was established. Three sets of primers were designed to amplify the event-specific fragments of GM maize events(Ly038,Mon863 and Mon810). Multiplex PCR and the separation of CE were systematically optimized,8.0g/L hydroxyethylcellulose in tris-phosphate-EDTA(TBE)buffer as sieving matrix,three event-specific multiplex PCR products were dete... |
| Keywords: | genetically modified maize multiplex polymerase chain reaction capillary electrophoresis UV detection |
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