| 有机磷农药三唑磷单克隆抗体制备及鉴定 |
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| 引用本文： | 韦倩妮,黄魁英,雷红涛,樊晓博,孙远明,徐振林,王弘,沈玉栋,徐怀德.有机磷农药三唑磷单克隆抗体制备及鉴定[J].食品科学,2011(1). |
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| 作者姓名： | 韦倩妮 黄魁英 雷红涛 樊晓博 孙远明 徐振林 王弘 沈玉栋 徐怀德 |
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| 作者单位： | 华南农业大学食品学院广东省食品质量安全重点实验室;广州市微生物研究所;西北农林科技大学食品科学与工程学院; |
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| 基金项目： | 国家自然科学基金项目(3070066320877029); 广东省高等学校科技成果转化项目(cgzhzd0808); 广东省科技计划项目(2009B040500002) |
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| 摘 要： | 采用活泼酯法和混合酸酐法将三唑磷半抗原(TZPM-Hap)与牛血清蛋白(BSA)和卵血清蛋白(OVA)偶联,分别制备出一种免疫抗原(TZPM-A-BSA)和两种包被抗原(TZPM-A-OVA和TZPM-M-OVA)。通过免疫小鼠及细胞融合,并对杂交瘤细胞进行经多次筛选和亚克隆得到1株产三唑磷单克隆抗体阳性细胞株(FC3),其抗体亚类为IgM。辛酸-硫酸铵沉淀法纯化后的抗体经间接ELISA检测,该方法IC50=9.7μg/L,最低检测限(LOD)为1.0μg/L,定量检测线性范围(IC20～IC80)为2.5～100.0μg/L,与其他有机磷农药结构类似物无明显交叉反应,显示出高度的特异性。该抗体可进一步用于免疫分析试剂盒开发。
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| 关 键 词： | 三唑磷 单克隆抗体 酶联免疫吸附检测 |
Production and Identification of Monoclonal Antibodies against Organophosphate Insceticide Triazophos |
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| WEI Qian-ni,HUANG Kui-ying,LEI Hong-tao,FAN Xiao-bo,SUN Yuan-ming,XU Zhen-lin,WANG Hong,SHEN Yu-dong,XU Huai-de.Production and Identification of Monoclonal Antibodies against Organophosphate Insceticide Triazophos[J].Food Science,2011(1). |
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| Authors: | WEI Qian-ni HUANG Kui-ying LEI Hong-tao FAN Xiao-bo SUN Yuan-ming XU Zhen-lin WANG Hong SHEN Yu-dong XU Huai-de |
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| Affiliation: | WEI Qian-ni1,HUANG Kui-ying2,LEI Hong-tao1,FAN Xiao-bo3,SUN Yuan-ming1,XU Zhen-lin1,WANG Hong1,SHEN Yu-dong1,XU Huai-de3(1.Key Laboratory of Food Quality and Safety of Guangdong Province,College of Food Science,South China Agricultural University,Guangzhou 510642,China,2.Guangdong Institute of Microbiology,Guangzhou 510663,3.College of Food Science,Northwest A&F University,Yangling 712100,China) |
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| Abstract: | In this study,artificial antigens for organophosphate insecticide triazophos including one immunogen and two coating antigens were prepared.The hapten(TZPM-Hap) of organophosphate insecticide triazophos was coupled to bovine serum albumin(BSA) by the active ester method to prepare the artificial immunogen TZPM-A-BSA.Coupling of TZPM-Hap to ovalbumin(OVA) by the active ester method or the mixed anhydride method produced the two coating antigens TZPM-A-OVA and TZPM-M-OVA,respectively.Balb/c mice were immunize... |
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| Keywords: | triazophos monoclonal antibody enzyme-linked immunosorbnent assay |
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