| 响应面法优化生物酶法合成植物甾醇酯 |
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| 引用本文： | 王腾宇,王铭义,宋玉卿,胡立志,于殿宇.响应面法优化生物酶法合成植物甾醇酯[J].食品科学,2011,32(4):59-63. |
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| 作者姓名： | 王腾宇 王铭义 宋玉卿 胡立志 于殿宇 |
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| 作者单位： | 1.东北农业大学食品学院 2.哈尔滨理工大学测控技术与通信工程学院 |
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| 基金项目： | 国家“863”计划项目(2010AA101503); 黑龙江省教育厅科学技术研究项目(11551064) |
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| 摘 要： | 采用Novozym 435脂肪酶催化大豆油与植物甾醇的酯交换反应，对其技术参数和条件进行探索。通过单因素与响应面分析，确定最佳工艺参数：反应温度101℃、酶用量1%、甾醇添加量5.2%、反应时间3h。在最佳条件下酯交换反应的转换率高达85.67%。
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| 关 键 词： | 植物甾醇 大豆油 酯交换反应 脂肪酶 |
Applying Response Surface Methodology to Optimize the Lipase-catalyzed Synthesis of Phytosterol Ester |
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| WANG Teng-yu,WANG Ming-yi,SONG Yu-qing,HU Li-zhi,YU Dian-yu.Applying Response Surface Methodology to Optimize the Lipase-catalyzed Synthesis of Phytosterol Ester[J].Food Science,2011,32(4):59-63. |
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| Authors: | WANG Teng-yu WANG Ming-yi SONG Yu-qing HU Li-zhi YU Dian-yu |
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| Affiliation: | 1. School of Food, Northeast Agricultural University, Harbin 150030, China；
2. School of Measurement-control Technology and Communication Engineering, Harbin University of Science and
Technology, Harbin 150086, China |
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| Abstract: | Transesterification was conducted in soybean oil with phytosterol under the catalysis of Novozym 435 lipase. The optimum reaction conditions were obtained by single factor experiments and response surface analysis to be reaction between soybean oil and added phytosterol at a concentration of 5.2% for 3 h at 101 ℃ in the presence of 1% lipase. The rate of transesterification was 85.67% under these conditions. |
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| Keywords: | phytosterol soybean oil transesterification lipase |
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