白藜芦醇对KKAy 小鼠的胰岛素敏感性影响及机制

目的：观察白藜芦醇对胰岛素抵抗小鼠(KKAy)胰岛素敏感性的影响，并探讨其机制。方法：将36 只8周龄KKAy 小鼠分成3 组，其中两组KKAy 小鼠分别饲喂添加白藜芦醇的AIN93G 饲料(2g/kg 饲料 或4g/kg 饲料)，另一组KKAy 小鼠为模型组，另外以12 只同周龄C57BL/6J 小鼠为对照。观察白藜芦醇对小鼠血糖、血清胰岛素 水平、葡萄糖耐量、胰岛素耐量等的影响；Western blot 实验检测不同组小鼠肝脏中沉默信息调节因子1(silent information regulator 1，sirt-1)蛋白表达及胰岛素受体底物1(insulin receptor substrate 1，IRS-1)的磷酸化水平。结 果：白藜芦醇干预可以增强KKAy 小鼠胰岛素敏感性，改善胰岛素耐量和葡萄糖耐量；肝脏中sirt-1 的表达和IRS-1 的磷酸化水平均升高。结论：白藜芦醇可以改善小鼠胰岛素抵抗及糖耐量异常，这可能与白藜芦醇提高肝脏中sirt-1 的表达，从而激活胰岛素信号通路相关蛋白有关。

Effect and Mechanism of Resveratrol on Insulin Sensitivity in Insulin-resistant KKAy Mice

Objective:To investigate the effect of resveratrol on insulin sensitivity in insulin-resistant KKAy mice and to probe the mechanism.Methods:A total of 36 7-week-old KKAy mice were randomly divided into 3 groups,which were fed standard AIN93G diet or standard AIN93G diet with resveratrol at contents of 2 g/kg and 4 g/kg diet.Totally 12 C57BL/6J mice were used as the control.The 12 h fasting levels of blood glucose and serum insulin and glucose and insulin tolerance at 0,6 weeks and 12 weeks of drug intervent...