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| 青藏高原藏木香总黄酮提取工艺研究及含量测定 | |
| 引用本文： | 杨月琴,彭敏,胡凤祖,常小平,马海乐,马世震. 青藏高原藏木香总黄酮提取工艺研究及含量测定[J]. 食品工业科技, 2011, 32(2): 251-253 |
| 作者姓名： | 杨月琴 彭敏 胡凤祖 常小平 马海乐 马世震 |
| 作者单位： | 1. 中国科学院西北高原生物研究所,青海西宁,810008;中国科学院研究生院,北京,100049;河南科技大学,河南洛阳,471003 2. 中国科学院西北高原生物研究所,青海西宁,810008 3. 青海省大通宝库林场,青海大通,810100 4. 河南科技大学,河南洛阳,471003 |
| 基金项目： | 河南科技大学特聘教授科研启动费(09004004); 青海省重点攻关项目(2006-034) |
| 摘 要： | 目的:研究藏木香中总黄酮的提取工艺及含量测定。方法:采用正交实验进行优选,考察乙醇浓度、溶剂倍数、提取时间等因素对黄酮得率的影响,用紫外分光光度法测定其含量。结果:乙醇浓度是主要的影响因素,最佳提取工艺为选用75%乙醇作为提取溶剂,料液比1∶20,水浴热回流提取2h。以芦丁为对照品,采用分光光度法测定出总黄酮平均含量为0.28mg/g。结论:提取工艺可行;紫外分光光度法简单、快速、准确;为藏木香深度开发提供了科学依据。 |
| 关 键 词： | 藏木香 黄酮 提取工艺 紫外分光光度法 |
Study on the extraction technique and quantification of total flavonoids in Inula racemosa grown in Qinghai-Tibet plateau |
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| YANG Yue-qin,PENG Min,HU Feng-zu,CHANG Xiao-ping,MA Hai-le,MA Shi-zhen. Study on the extraction technique and quantification of total flavonoids in Inula racemosa grown in Qinghai-Tibet plateau[J]. Science and Technology of Food Industry, 2011, 32(2): 251-253 | |
| Authors: | YANG Yue-qin PENG Min HU Feng-zu CHANG Xiao-ping MA Hai-le MA Shi-zhen |
| Affiliation: | YANG Yue-qin1,2,3,PENG Min1,HU Feng-zu1,CHANG Xiao-ping4,MA Hai-le3,MA Shi-zhen1 (1. Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China,2. Graduate University of Chinese Academy of Sciences,Beijing 100049,3.Henan University of Science and Technology,Luoyang 471003,4. Qinghai Baoku Forestry Farm,Datong 810100,China) |
| Abstract: | Objective:Aim to study the best conditions of extraction techniques for total flavonoids in Inula racemosa. Methods:The best conditions of extraction technique for total flavonoids were optimized with orthogonal experiment. Developed by ethanol solution with different concentrations,solvent multiples and extraction duration,determination of total flavonoids with UV-spectrophotometry was made. Results:Ethanol concentration was the major factor for determining total flavonoids contents. The best conditions fo... |
| Keywords: | Inula racemosa flavonoids extraction technique UV-spectrophotometric method |
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