| 高产酒精酵母菌株及甘薯品种筛选 |
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| 引用本文： | 聂世现,王钰,黄文静,季必霞,叶亚峰,刘小平. 高产酒精酵母菌株及甘薯品种筛选[J]. 食品工业科技, 2011, 32(2): 159-161,235 |
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| 作者姓名： | 聂世现 王钰 黄文静 季必霞 叶亚峰 刘小平 |
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| 作者单位： | 1. 安徽大学生命科学学院,安徽省生态工程与生物技术重点实验室,安徽合肥230039
2. 安徽省农科院作物研究所,安徽合肥,230031 |
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| 基金项目： | 安徽省教育厅重点项目(KJ2008A077) |
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| 摘 要： | 以酒曲中筛选到的酵母菌A4为出发菌株,用紫外线对其原生质体进行物理诱变,经过三级筛选得到突变高产菌株A4-20。以麦芽汁为基质发酵72h后,酒精度为4.93%,较出发菌株提高了28.7%。经过连续转接10代,A4-20的发酵性能表现稳定。用其对6种高淀粉品种甘薯淀粉进行发酵实验,得出3个品种的酒精产量均达14%以上,其中徐薯22酒精产量达到最高14.87%,出酒率为38.1%,经比较,徐薯22、皖薯3号和199031-1更适合应用于酒精产业。
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| 关 键 词： | 原生质体 紫外线诱变 甘薯 酒精 发酵 |
Screening of high yield alcohol yeast and sweet potato varieties |
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| NIE Shi-xian,WANG Yu,HUANG Wen-jing,JI Bi-xia,YE Ya-feng,LIU Xiao-ping. Screening of high yield alcohol yeast and sweet potato varieties[J]. Science and Technology of Food Industry, 2011, 32(2): 159-161,235 |
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| Authors: | NIE Shi-xian WANG Yu HUANG Wen-jing JI Bi-xia YE Ya-feng LIU Xiao-ping |
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| Affiliation: | NIE Shi-xian1,WANG Yu1,HUANG Wen-jing1,JI Bi-xia1,YE Ya-feng1,LIU Xiao-ping2 (1.Provincial Key Laboratory of Eco-engineering and Bio-technique,College of Life Science,Anhui University,Hefei 230039,China,2. Crop Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China) |
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| Abstract: | The current study was to select A4 from distillers yeast as original strain. Strain A4-20 was screened as a high-alcohol-yield strain by UV-mutation of protoplast and three steps screening method. Through wort fermentation for 72h,the alcohol content reached 4.93%,which was 28.7% higher than the original stain. After switched over 10 generations,and the fermentation by A4-20 was not significant difference. Using A4-20 fermented the starch of 6 high starch varieties of sweet potato,the results showed that th... |
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| Keywords: | protoplast UV-mutation sweet potato alcohol fermentation |
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