鲜玉米中可溶性糖含量的测定

采用高效液相色谱法对鲜玉米中可溶性糖含量进行检测。色谱柱为Agilent Zorbax carbohydrate柱(4.6mm×250mm，5μm)，流动相为75%乙腈，柱温25℃，流速1mL/min。相关系数r在0.99839～0.99995(n=5)之间；精密度实验RSD为0.90%～3.80%(n=5)；平均回收率在95.28%～101.02%之间。该方法准确、可靠，可以快速准确的对鲜玉米中可溶性糖进行定性定量分析。

Determination of Soluble Sugar Content in Fresh Corn

A high performance liquid chromatography (HPLC) method was used to determine the contents three soluble sugars (fructose, sucrose and glucose) in fresh corn. The chromatographic column used was an Agilent Zorbax carbohydrate (4.6 mm × 250 mm, 5 μm) column held at 25 ℃, and the mobile phase was a mixture of acetonitrile and water (V/V, 75:25) at a flow rate of 1 mL/min. The established cabliration curves of fructose, sucrose and glucose displayed correlation coefficients ranging from 0.99839 to 0.99995 (n = 5). In precision experiments, relative standard deviations (RSDs) between 0.90% and 3.8% were obtained (n = 5). The average spike recoveries of the method ranged from 95.28% to 101.02%. This method can be used for accurate, reasonable and rapid analysis of soluble sugar in fresh corn.