| 保健食品铁皮石斛及其混淆品的分子鉴别及亲缘关系分析 |
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| 引用本文： | 丁鸽,张代臻,张伟超,丁小余. 保健食品铁皮石斛及其混淆品的分子鉴别及亲缘关系分析[J]. 食品科学, 2011, 32(2): 141-145. DOI: 10.7506/spkx1002-6630-201102033 |
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| 作者姓名： | 丁鸽 张代臻 张伟超 丁小余 |
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| 作者单位： | 1. 盐城工学院化学与生物工程学院2. 南京师范大学生命科学学院3. 江苏省滩涂生物资源与环境保护重点建设实验室 4.环境保护部南京环境科学研究所 |
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| 基金项目： | 国家自然科学基金项目(31000142;30870234); 盐城工学院博士启动基金项目;盐城工学院科研项目基金项目(XKR2010003) |
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| 摘 要： | 目的：寻找简易且重复性高的分子标记方法对保健食品铁皮石斛及其混淆品进行分子鉴别并对其亲缘关系进行分析。方法：对铁皮石斛及其混淆品的matK 基因进行扩增、测序并进行序列分析，寻找其中存在的单核苷酸多态性(SNP)位点并根据SNP 位点设计特异性鉴别引物；运用Mega 3.0 等软件进行遗传关系分析。结果：根据matK 基因序列中存在的SNP 位点及特异性鉴别引物，可将铁皮石斛与其混淆品成功分开；各样品之间存在较大的遗传差异。结论：SNP 位点及特异性鉴别引物可对保健食品铁皮石斛及其混淆品进行快速、简便的鉴别，通过matK 基因可以分析正品与混淆品之间的亲缘关系。
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| 关 键 词： | 铁皮石斛 matK 基因 单核苷酸多态性 位点特异性聚合酶链式反应(PCR) 分子鉴别 |
| 收稿时间： | 2010-03-05 |
Molecular Identification and Phylogenetic Analysis of Functional Food Dendrobium officinale and Its Adulterants |
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| DING Ge,ZHANG Dai-zhen,ZHANG Wei-chao,DING Xiao-yu. Molecular Identification and Phylogenetic Analysis of Functional Food Dendrobium officinale and Its Adulterants[J]. Food Science, 2011, 32(2): 141-145. DOI: 10.7506/spkx1002-6630-201102033 |
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| Authors: | DING Ge ZHANG Dai-zhen ZHANG Wei-chao DING Xiao-yu |
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| Affiliation: | 1. Chemical and Biological Engineering College, Yancheng Institute of Technology, Yancheng 224003, China；2. College of Life Science, Nanjing Normal University, Nanjing 210046, China；3. Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng 224002, China；4. Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, China |
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| Abstract: | To develop a simple and reproducible molecular method to discriminate between functional food Dendrobium officinale and its adulterants,PCR products of the matK gene were sequenced to find single nucleotide polymorphism(SNP)sites.Then allele-specific PCR primers were designed according to the identified SNP sites.The phylogeny of D.officinale and its adulterants were analyzed by Mega 3.0.It was found that D.officinale and its adulterants can be discriminated by SNP sites and allele-specific PCR primers.Ther... |
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| Keywords: | Dendrobium officinale matK gene single nucleotide polymorphism allele-specific PCR molecular discrimination |
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