| 利用衍生化试剂DTNB测定枸杞中谷胱甘肽 |
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| 引用本文： | 朱亚玲,张小勇,崔胜云.利用衍生化试剂DTNB测定枸杞中谷胱甘肽[J].食品科学,2011(6). |
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| 作者姓名： | 朱亚玲 张小勇 崔胜云 |
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| 作者单位： | 延边大学长白山生物资源与功能分子教育部重点实验室; |
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| 摘 要： | 利用5,5'-二硫硝基苯甲酸(DTNB)衍生化试剂和植物材料同步破壁提取的样品处理方法,采用电喷雾质谱法和分光光度法对枸杞中还原型谷胱甘肽(GSH)进行定性、定量分析。枸杞样品提取过程中GSH受破壁后胞外化学环境的影响导致氧化变性。利用5,5'-二硫硝基苯甲酸(DTNB)衍生化试剂同步提取,因生成稳定的GSH的衍生化产物避免了GSH氧化变性导致的GSH分析信息丢失。用UV-Vis分光光度法测定GSH含量,发现枸杞中含有丰富的GSH。标准加入法测定枸杞干品中GSH,其含量范围达(6.56±0.54)mg/g,是目前所测植物样品中GSH含量较高的物种。本研究对药用植物胞内活性物质的准确分析提供方法学的尝试,并通过测定发现枸杞中富含GSH。
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| 关 键 词： | 谷胱甘肽 5 5'-二硫硝基苯甲酸 枸杞 分光光度法 质谱分析法 同步衍生化 |
Determination of GSH in Lycium barbarum L Fruits by Derivatizing Agent |
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| ZHU Ya-ling,ZHANG Xiao-yong,CUI Sheng-yun.Determination of GSH in Lycium barbarum L Fruits by Derivatizing Agent[J].Food Science,2011(6). |
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| Authors: | ZHU Ya-ling ZHANG Xiao-yong CUI Sheng-yun |
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| Affiliation: | ZHU Ya-ling,ZHANG Xiao-yong,CUI Sheng-yun(Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules,Ministry of Education,Yanbian University,Yanji 133002,China) |
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| Abstract: | The content of reduced glutathione(GSH) in Lycium barbarum L fruits was determined by mass spectroscopy and UV-visible spectroscopy using 5,5'-dithio-bis-nitrobenzoic acid(DTNB) as the simultaneous derivatizing agent.GSH in Lycium barbarum L fruits was significantly affected by extracellular chemical environment during sample treatment.Extraction with DTNB as the simultaneous derivatizing agent inhibited the transformation or decomposition of GSH due to immediate derivatization during sample treatment.Quant... |
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| Keywords: | glutathione 5 5'-dithio-bis-nitrobenzoic acid(DTNB) Lycium barbarum L mass spectroscopy simultaneous derivatization |
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