| 瓶装泥螺和蟹糊可培养细菌的分离与鉴定 |
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| 引用本文： | 黄键,陈燕,全晶晶,张春丹,李晔,裘迪红,汤海青,李和生,苏秀榕,章超桦,秦小明.瓶装泥螺和蟹糊可培养细菌的分离与鉴定[J].食品科学,2011(5). |
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| 作者姓名： | 黄键 陈燕 全晶晶 张春丹 李晔 裘迪红 汤海青 李和生 苏秀榕 章超桦 秦小明 |
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| 作者单位： | 宁波大学生命科学与生物工程学院;广东海洋大学食品科技学院; |
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| 基金项目： | 国家现代农业产业技术体系建设专项(nycy-47); 浙江省重中之重学科资助项目(XK0613040); 浙江省教育厅重点资助项目(200517011) |
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| 摘 要： | 为提高泥螺和蟹糊的贮藏时间,确保生食的食用安全性,本实验利用常规方法从瓶装泥螺中分离和鉴定出3株细菌,分别是弯曲芽孢杆菌(Bacillus flexus)、绿色气球菌(Aerococcus viridans)和乳酪短杆菌(Brevibacteriumcasei);从瓶装蟹糊中分离和鉴定出的4株细菌分别为:鸡葡萄球菌(Staphylococcus gallinarum)、卡他莫拉菌(Moraxella catarrhalis)、藤黄微球菌(Micrococcus luteus)和纤细发光杆菌(Photobacterium angustum)。16S rRNA鉴定鉴定结果表明只有纤细发光杆菌的鉴定结果与MIS鉴定的结果不一致。除卡他莫拉菌、绿色气球菌和乳酪短杆菌外,其余菌都随着温度升高,长势增强。7株细菌中除卡他莫拉菌在低温冷藏(4℃)条件下长势明显增加外,其余菌都在低温冷藏(4℃)条件下长势明显减弱。7株菌都具有胶原蛋白酶和酪蛋白酶活性,藤黄微球菌和纤细发光杆菌不能分解利用淀粉,所有的分离菌株都没有酯酶活性。这些菌的生长温度范围为4～35℃(最适温度35℃),盐度范围为3%～7%(最适盐度3%),pH值范围5....
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| 关 键 词： | 泥螺 蟹糊 细菌自动鉴定 16S rRNA PCR扩增 |
Isolation and Identification of Cultivable Microorganisms in Bottled Crab Paste and Salted Mudsnail |
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| HUANG Jian,CHEN Yan,QUAN Jing-jing,ZHANG Chun-dan,LI Ye,QIU Di-hong,TANG Hai-qing,LI He-sheng,SU Xiu-rong,ZHANG Chao-hua,QIN Xiao-ming.Isolation and Identification of Cultivable Microorganisms in Bottled Crab Paste and Salted Mudsnail[J].Food Science,2011(5). |
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| Authors: | HUANG Jian CHEN Yan QUAN Jing-jing ZHANG Chun-dan LI Ye QIU Di-hong TANG Hai-qing LI He-sheng SU Xiu-rong ZHANG Chao-hua QIN Xiao-ming |
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| Affiliation: | HUANG Jian1,CHEN Yan1,QUAN Jing-jing1,ZHANG Chun-dan1,LI Ye1,QIU Di-hong1,TANG Hai-qing1,LI He-sheng1,SU Xiu-rong1,*,ZHANG Chao-hua2,QIN Xiao-ming2(1.Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China,2.College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,China) |
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| Abstract: | In order to increase the storage time and ensure edible safety of crab paste and salted mudsnail,bacterial analysis of crab paste and salted mudsnail was conducted.Three bacterial strains were isolated from salted mudsnail and identified as Bacillus flexus,Aerococcus viridans and Brevibacterium casei.Similarly,4 bacterial strains were isolated from crab paste and identified as Staphylococcus gallinarum,Moraxella catarrhalis,Micrococcus luteus and Photobacterium angustum.In addition,highly consistent results... |
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| Keywords: | salted mudsnail crab paste bacterial automatic identification 16S rRNA PCR amplification |
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