昆明系小鼠生物法定量测定水产品中河豚毒素

为建立昆明系小鼠定量测定河豚毒素(TTX)方法，选用体质量19～21g的昆明系小鼠作为实验对象，用不同质量浓度TTX溶液腹腔注射小鼠。结果显示：TTX致昆明系小鼠的半数致死量(LD50)为0.18μg，小鼠死亡时间与TTX注射剂量满足方程Y=e(0.357/X＋1.005)。此外，采用体积分数0.5%乙酸溶液提取TTX，回收率达到90%以上，对样品的最低检测限为0.56μg/g。昆明系小鼠定量测定河豚毒素，准确、稳定、方便快捷，可用于水产品安全检测。

Quantitative Detection of Tetrodotoxin Using Kunming Strain Mice

This study was aimed at establishing a laboratory method for the quantitative detection of tetrodotoxin (TTX). A bioassay was developed by injecting intraperitoneally TTX at various dosages to Kunming strain male mice with body weights of 19－21 g. Results indicated that the 50% lethal dose (LD50) of TTX for Kunming strain mice was 0.18μg. The relationship between TTX dosage and the mean death time of Kunming mice could be expressed as Y = e(0.357/ X +1.005). In addition, a high recovery rate of TTX was achieved by using 0.5% (V/V) acetic acid to extract TTX from globefish, which reached up to 90%. However, the detection limit of the assay for samples was 0.56μg/g. Therefore, Kunming strain mice can be used to quantitatively determine TTX in an accurate, stable and convenient manner, which is promising in the safety control of fishery products.