| 高效液相色谱-串联质谱测定猪肝中阿维菌素、伊维菌素残留 |
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| 引用本文： | 郑卫东,胡江涛,阴文娅,盛毅,武志雄.高效液相色谱-串联质谱测定猪肝中阿维菌素、伊维菌素残留[J].食品科学,2011,32(4):185-188. |
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| 作者姓名： | 郑卫东 胡江涛 阴文娅 盛毅 武志雄 |
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| 作者单位： | 1.四川省产品质量监督检验检测院 2.四川出入境检验检疫局 3.四川大学华西公共卫生学院营养与食品卫生学教研室 |
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| 基金项目： | 四川省科技厅应用基础研究项目(04JY029-033) |
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| 摘 要： | 目的：建立高效液相色谱-四极杆串联质谱仪(LC-MS/MS)同时测定猪肝脏中阿维菌素(AVM)和伊维菌素(IVM)的残留量。方法：猪肝样品均质后用乙腈提取，碱性氧化铝固相萃取柱(SPE)净化，采用串联质谱电喷雾电离ESI(+)，多反应监测模式(MRM)检测，以保留时间和子离子比定性，外标法定量。结果：方法检出限(LOD)均为2.0μg/kg，定量限(LOQ)为5.0μg/L，两种药物的线性范围均为5～100μg/L，相关系数均为0.9999。添加水平在2～20μg/kg范围内，阿维菌素和伊维菌素的平均回收率分别为77.0%～83.3%和76.9%～79.8%之间，相对标准偏差(RSD)分别小于12.1%和13.0%。结论：方法操作简单，灵敏准确，可满足当前国内外该产品中此类药物的检测要求。
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| 关 键 词： | 猪肝 阿维菌素 伊维菌素 高效液相色谱-串联质谱 |
Determination of Avermectin and Ivermectin Residues in Pork Liver by High Performance Liquid Chromatography-Mass Spectrometry |
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| ZHENG Wei-dong,HU Jiang-tao,YIN Wen-ya,SHENG Yi,WU Zhi-xiong.Determination of Avermectin and Ivermectin Residues in Pork Liver by High Performance Liquid Chromatography-Mass Spectrometry[J].Food Science,2011,32(4):185-188. |
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| Authors: | ZHENG Wei-dong HU Jiang-tao YIN Wen-ya SHENG Yi WU Zhi-xiong |
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| Affiliation: | 1. Sichuan Institute of Product Quality Supervision and Inspection, Chengdu 610031, China；
2. Sichuan Entry-exit Inspection and Quarantine Bureau, Chengdu 610041, China；
3. Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China |
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| Abstract: | Objective:To establish a method for the simultaneous determination of avermectin and ivermectin residues in pork liver using high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Methods:Avermectin and ivermectin residues were extracted with acetonitrile,cleaned up on a solid phase extraction (SPE) column containing alkaline aluminum oxide,separated on a C8 column,detected by tandem mass spectrometry with electrospray ionization and multiple reaction monit... |
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| Keywords: | pork liver avermectin ivermectin high performance liquid chromatography-tandem mass spectrometry |
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