| 百合粉中秋水仙碱的提取及抑菌性研究 |
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| 引用本文： | 陈莉华,张丽,徐果.百合粉中秋水仙碱的提取及抑菌性研究[J].食品科学,2011,32(6):57-60. |
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| 作者姓名： | 陈莉华 张丽 徐果 |
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| 作者单位： | 1.吉首大学化学化工学院 2.吉首大学生物资源与环境科学学院 |
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| 基金项目： | 科技部科技型中小企业技术创新基金项目(10C26214302421) |
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| 摘 要： | 目的：研究百合粉中秋水仙碱的提取方法及抑菌作用。方法：用有机溶剂从百合粉中提取秋水仙碱，用高效液相色谱法测定提取物中秋水仙碱含量，通过单因素和正交试验对提取条件进行优化筛选，确定适宜的工艺参数，测定秋水仙碱对大肠杆菌、金黄色葡萄球菌、枯草杆菌、白念球菌、藤黄八叠球菌的体外最小抑菌质量浓度(MIC)及抑菌率。结果：提取百合粉中秋水仙碱的最佳提取条件为温度80℃、95%乙醇料液比1:6混合、提取8h；秋水仙碱对大肠杆菌、金黄色葡萄球菌、枯草杆菌、白念球菌、藤黄八叠球菌的MIC分别为0.20、0.20、0.24、0.16、0.20mg/mL。结论：百合粉中的秋水仙碱对这5种菌都有明显的抑制作用。
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| 关 键 词： | 秋水仙碱 百合粉 高效液相色谱 最小抑菌浓度 抑菌率 |
| 收稿时间： | 2010-06-12 |
Extraction and Antibacterial Activity of Colchicine from Lily Root Powder |
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| CHEN Li-hua,ZHANG Li,XU Guo.Extraction and Antibacterial Activity of Colchicine from Lily Root Powder[J].Food Science,2011,32(6):57-60. |
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| Authors: | CHEN Li-hua ZHANG Li XU Guo |
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| Affiliation: | 1. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China；
2. College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China |
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| Abstract: | Objective:To explore the extraction and antibacterial activity of colchicine from lily root powder.Methods:Colchicine was extracted from the dry powder of lily roots.The content of colchicine in the extract was determined by using high performance liquid chromatography(HPLC).The optimal extraction conditions were explored by single factor and orthogonal array design methods.Minimal inhibitory concentration(MIC) and antibacterial rate of colchicine were measured by bacteriostasis tests in vitro.Results:The o... |
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| Keywords: | colchicines lily root powder HPLC minimal inhibitory concentration antibacterial rate |
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