响应曲面法优化尿素包合富集蚕蛹油α-亚麻酸的工艺

采用响应曲面法对尿素包合富集蚕蛹油α-亚麻酸工艺进行优化。以α-亚麻酸纯度为响应值，温度、尿素与脂肪酸比值、95%乙醇与脂肪酸比值和包合时间为试验因素，进行中心组合试验。结果表明，α-亚麻酸富集最优工艺条件为温度－8.27℃、尿素与脂肪酸比值3.39、95%乙醇与脂肪酸比值10.47、包合时间8.91h，α-亚麻酸纯度达70.28%。尿素包合法是富集蚕蛹油α-亚麻酸有效的方法。

Optimization of the Enrichment of Alpha-Linolenic Acid in Silkworm Pupal Oil by the Method of Urea Adduction Fractionation

Response surface methodology (RSM) was applied to optimize the enrichment of α-linolenic acid in silkworm pupal oil by the method of urea adduction fractionation. This optimization was carried out based on our previous investigations on the effects of four process parameters, namely reaction temperature and time, the ratio of urea to fatty acids, and the ratio of 95% ethanol to fatty acids on α-linolenic acid content in urea inclusion compounds by single factor method, and the results showed that the optimum levels of the four parameters were determined to be －8.27 ℃, 8.91 h, 3.39:1 and 10.47:1, respectively. Under the optimum conditions, the content of α-linolenic acid in urea inclusion compounds was up to 70.28%. Urea adduction fractionation is an effective method for the enrichment of α-linolenic acid.