Protective effect of vitamin E on linoleic acid hydroperoxide-induced injury to human endothelial cells |
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Authors: | Takao Kaneko Shun-ichiro Nakano Mitsuyoshi Matsuo |
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Affiliation: | (1) Tokyo Metropolitan Institute of Gerontology, 35-2 Sakaecho, Itabashiku, 173 Tokyo, Japan |
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Abstract: | The protective effects of vitamin E (α-tocopherol) and its model compounds, which act as antioxidants, on linoleic acid hydroperoxide-induced injury to human umbilical vein endothelial cells were examined. When incubated at 50 μM with endothelial cells at 37°C for 24 hr, α-tocopherol protected the cells from injury, and 2,2,5,7,8-pentamethylchroman-6-ol showed a similar protective effect. Trolox C, a water-soluble vitamin E model compound, had no protective effect. Tocol, a poor antioxidant, proved toxic. During preincubation, α-tocopherol was incorporated into the cells at 16.6 nmol/ mg protein, while the pentamethylchromanol was incorporated at 0.5 nmol/mg protein; Trolox C was not incorporated at all. The results suggest that agents having both high antioxidant activity and lipophilicity can protect endothelial cells from linoleic acid hydroperoxide-induced injury. Issued as TMIG-I No. 170. |
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