The synthesis and degradation of collagenase-degradable poly(2-hydroxyethyl methacrylate)-based hydrogels and sponges for potential applications as scaffolds in tissue engineering |
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| Authors: | Stefan M. Paterson Audra M.A. Shadforth David H. Brown Peter W. Madden Traian V. Chirila Murray V. Baker |
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| Affiliation: | 1. School of Chemistry and Biochemistry, M313, The University of Western Australia, Crawley, W.A. 6009, Australia;2. Queensland Eye Institute, 41 Annerley Road, South Brisbane, Queensland 4101, Australia;3. Nanochemistry Research Institute, Department of Chemistry, Curtin University, Kent St, Bentley, W.A. 6102, Australia;4. Science and Engineering Faculty, Queensland University of Technology, Brisbane, Queensland 4001, Australia;5. Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St Lucia, Queensland 4072, Australia;6. Faculty of Health Sciences, University of Queensland, Herston, Queensland 4006, Australia |
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| Abstract: | A collagenase-cleavable peptide-based crosslinking agent was synthesized and was incorporated into PHEMA sponges, and P[HEMA-co-MeO-PEGMA] gels and sponges [HEMA 2-hydroxyethyl methacrylate, PHEMA = poly(2-hydroxyethyl methacrylate), MeO-PEGMA = poly(ethylene glycol) monomethyl ether methacrylate]. PHEMA and P[HEMA-co-MeO-PEGMA] sponges had polymer droplet morphologies where the dimensions of the morphological features were three to five times larger compared to sponges that were crosslinked with tetraethylene glycol dimethacrylate (TEGDMA), while the P[HEMA-co-MeO-PEGMA] gels had similar morphologies regardless of the crosslinking agent. The differences in the dimensions of the morphologies of the sponges were attributed to differences in hydrophilicities of the crosslinking agent. When incubated in a collagenase solution, PHEMA sponges did not degrade, but P[HEMA-co-MeO-PEGMA] gels took 28 days to degrade and the P[HEMA-co-MeO-PEGMA] sponges took 101 days to degrade to 8% dry weight remaining. A cytotoxicity assay showed that the hydrogels do not elicit any cytotoxic response in vitro. |
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