Fibroblast functionality on novel Ti30Ta nanotube array |
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Authors: | Patricia Capellato Barbara S Smith Ketul C Popat Ana PR Alves Claro |
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Affiliation: | 1. Department of Materials, Faculty of Engineering Guaratinguetá, Sao Paulo State University—UNESP, Av. Ariberto Pereira da Cunha, 333, Pedregulho, CEP 12516‐410, Guaratinguetá, SP, Brazil;2. School of Biomedical Engineering, Colorado State University, Fort Collins CO 80523, USA;3. Department of Mechanical Engineering, Colorado State University, Fort Collins, CO 80523, USA |
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Abstract: | In this study, the mechanical substrate and topographical surface properties of anodized Ti 30Ta alloy were investigated using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and contact angle measurement. The anodization process was performed in an electrolyte solution containing HF (48%) and H2SO4 (98%) in the volumetric ratios 1:9 with the addition of 5% dimethyl sulfoxide (DMSO) at 15 V, 25 V and 35 V for 20 and 40 min, producing a nanotube architecture when anodized at 35 V for 40 min. Human dermal fibroblasts (HDF, neonatal) were utilized to evaluate the biocompatibility of Ti 30Ta nanotubes and Ti 30Ta alloy after 1 and 3 days of culture. Cellular adhesion, proliferation, viability, cytoskeletal organization and morphology were investigated using fluorescence microscope imaging, biochemical assay and SEM imaging respectively. The results presented identify altered material properties and improved cellular interaction on Ti 30Ta nanotubes as compared to Ti 30 Ta alloy. |
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