Amperometric glucose sensor based on enhanced catalytic reduction of oxygen using glucose oxidase adsorbed onto core-shell Fe3O4@silica@Au magnetic nanoparticles |
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| Authors: | Ai-Jun Wang Yong-Fang Li Zhong-Hua Li Jiu-Ju Feng Yan-Li Sun Jian-Rong Chen |
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| Affiliation: | 1. College of Geography and Environmental Science, College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;2. College of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang 453003, China;3. Key Laboratory of Green Chemical Media and Reactions, Ministry of Education; School of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China |
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| Abstract: | Monodisperse Fe3O4 magnetic nanoparticles (NPs) were prepared under facile solvothermal conditions and successively functionalized with silica and Au to form core/shell Fe3O4@silica@Au NPs. Furthermore, the samples were used as matrix to construct a glucose sensor based on glucose oxidase (GOD). The immobilized GOD retained its bioactivity with high protein load of 3.92 × 10? 9 mol·cm? 2, and exhibited a surface-controlled quasi-reversible redox reaction, with a fast heterogeneous electron transfer rate of 7.98 ± 0.6 s? 1. The glucose biosensor showed a broad linear range up to 3.97 mM with high sensitivity of 62.45 μA·mM? 1 cm? 2 and fast response (less than 5 s). |
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