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Collagen peptides from Acaudina molpadioides prevent CCl4-induced liver injury via Keap1/Nrf2-ARE,PI3K/AKT,and MAPKs pathways |
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| Authors: | Jie Li Yan Li Saijun Lin Wei Zhao Yan Chen Huoxi Jin |
| Affiliation: | 1. Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China
Contribution: Data curation, Investigation;2. Hangzhou Institute for Food and Drug Control, Hangzhou, China Contribution: Resources, Software;3. Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China Contribution: Data curation;4. Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China Contribution: Funding acquisition, Project administration;5. Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China |
| Abstract: | Collagen peptide from Acaudina molpadioides (AMP) showed antioxidative activity in H2O2-induced RAW264.7 cells in our pervious study. In this study, it was observed that AMP could effectively improve the morphology and function of liver in CCl4-induced mice. After 200 mg/kg AMP treatment, the content of MDA in liver decreased by 62.3%, and the level of antioxidant enzymes (SOD, GSH-Px, and CAT) increased by more than 65%. Western blot results disclosed that AMP (200 mg/kg) upregulated the Nrf2 level by 73.8% and downregulated Keap1 by 41.0% in CCl4-induced mice liver. The levels of p-ERK, p-JNK, and p-p38 in 200 mg/kg AMP treatment groups decreased by 57.3%, 40.9%, and 40.6%, but the levels of p-PI3K and p-AKT increased by 162.6% and 60.3%, respectively. Furthermore, the trends of Nrf2, Keap1, p-ERK, p-JNK, p-p38, p-PI3K, and p-AKT levels in H2O2-induced RAW264.7 cells after AMP treatment were similar to the results in CCl4-induced mice liver. These findings provided evidence that AMP exerted antioxidant activity via Keap1/Nrf2-ARE, PI3K/AKT, and MAPKs pathways in vivo and in vitro. Therefore, the collagen peptide from A. molpadioides might represent a novel functional food to prevent acute liver injury via attenuation of oxidative stress. |
| Keywords: | Acaudina molpadioides collagen peptides Keap1/Nrf2-ARE liver injury PI3K/Akt |