Hyaluronan and fibrin biomaterial as scaffolds for neuronal differentiation of adult stem cells derived from adipose tissue and skin |
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Authors: | Gardin Chiara Vindigni Vincenzo Bressan Eriberto Ferroni Letizia Nalesso Elisa Puppa Alessandro Della D'Avella Domenico Lops Diego Pinton Paolo Zavan Barbara |
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Affiliation: | Department of Histology, Microbiology and Medical Biotechnology, University of Padova, Via G. Colombo 3, 35100 Padova, Italy; E-Mails: Chiaragardin@unipd.it (C.G.); letia.ferroni@unipd.it (L.F.); elisa.nalessoo@unipd.it (E.N.). |
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Abstract: | Recently, we have described a simple protocol to obtain an enriched culture of adult stem cells organized in neurospheres from two post-natal tissues: skin and adipose tissue. Due to their possible application in neuronal tissue regeneration, here we tested two kinds of scaffold well known in tissue engineering application: hyaluronan based membranes and fibrin-glue meshes. Neurospheres from skin and adipose tissue were seeded onto two scaffold types: hyaluronan based membrane and fibrin-glue meshes. Neurospheres were then induced to acquire a glial and neuronal-like phenotype. Gene expression, morphological feature and chromosomal imbalance (kariotype) were analyzed and compared. Adipose and skin derived neurospheres are able to grow well and to differentiate into glial/neuron cells without any chromosomal imbalance in both scaffolds. Adult cells are able to express typical cell surface markers such as S100; GFAP; nestin; βIII tubulin; CNPase. In summary, we have demonstrated that neurospheres isolated from skin and adipose tissues are able to differentiate in glial/neuron-like cells, without any chromosomal imbalance in two scaffold types, useful for tissue engineering application: hyaluronan based membrane and fibrin-glue meshes. |
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Keywords: | adipose derived stem cells skin adipose tissue stem cells Schwann cell karyotypes |
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